Synthesis and evaluation of a C-6 alkylated pyrimidine derivative for in vivo imaging of HSV1-TK gene expression (CROSBI ID 173658)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Müller, Ursina ; Martić, Miljen ; Gazivoda Kraljević, Tatjana ; Krištafor, Svjetlana ; Ranadheera, Charlene ; Müller, Adrienne ; Born, Mariana ; Krämer, Stefanie D. ; Raić-Malić, Silvana ; Ametamey, Simon M.
engleski
Synthesis and evaluation of a C-6 alkylated pyrimidine derivative for in vivo imaging of HSV1-TK gene expression
We report on the synthesis, radiolabeling, in vitro and in vivo characterization of N-Me-[18F]fluoro-2-(hydroxymethyl)propyl)-1, 5-dimethylpyrimidin-2, 4(1H, 3H-dione), a C-6 substituted N-1 methylated pyrimidine derivative as a reporter probe for imaging herpes simplex virus type 1 thymidine kinase (HSV1-TK) expression. N-Me-[18F]FHBT was synthesized via a standard nucleophilic substitution reaction followed by acidic cleavage of the methoxytrityl protecting group. Cell uptake was studied in vitro with control HEK293 (human embryonic kidney cells) and HEK293 cells stably transfected with non-mutant HSV1-tk (HEK293TK+ cells). PET imaging and biodistribution studies of N-Me-[18F]FHBT or [18F]FHBG were performed in nude mice bearing xenografts of HEK293 control and TK-positive cells. N-Me-[18F]FHBT (18F-5) was obtained in two step reaction in an overall maximal radiochemical yield (decay corrected) of 5 % and a radiochemical purity >96 %. The tracer uptake in HSV1-TK containing HEK293TK+ cells was 5.2 times (at 30 min) and 81.5 times (at 240 min) higher than that in control HEK293 cells. In mice, N-Me-[18F]FHBT and [18F]FHBG accumulated significantly and exhibited similar radioactivity in the HEK293TK+ xenografts, however, SUV ratios between HEK293TK+ and HEK293control xenografts were Higher for [18F]FHBG than for N-Me-[18F]FHBT. Both tracers showed gall bladder and abdominal activity. The biological evaluations demonstrated the feasibility of using N-methylated C-6 substituted pyrimidine derivative N-Me-[18F]FHBT as a PET radiotracer for monitoring HSV1-TK expression in vivo.
HSV1-TK ; reporter gene ; gene expression monitoring ; PET ; N-Me-FHBT ; FHBG
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Podaci o izdanju
39 (2)
2012.
235-246
objavljeno
0969-8051
1872-9614
10.1016/j.nucmedbio.2011.07.009