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Pregled bibliografske jedinice broj: 523532

Synthesis and study of nucleic acids interactions of novel monomethine cyanine dyes


Kaloyanova, Stefka; Crnolatac, Ivo; Lesev, Nedyalko; Piantanida, Ivo; Deligeorgiev, Todor
Synthesis and study of nucleic acids interactions of novel monomethine cyanine dyes // DYES AND PIGMENTS, 92 (2012), 3; 1184-1191 doi:10.1016/j.dyepig.2011.08.019 (međunarodna recenzija, članak, znanstveni)


Naslov
Synthesis and study of nucleic acids interactions of novel monomethine cyanine dyes

Autori
Kaloyanova, Stefka ; Crnolatac, Ivo ; Lesev, Nedyalko ; Piantanida, Ivo ; Deligeorgiev, Todor

Izvornik
DYES AND PIGMENTS (0143-7208) 92 (2012), 3; 1184-1191

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Cyanine dyes; DNA binding; fluorescence; thermal melting; circular dichroism

Sažetak
Six asymmetric monomethine cyanine dyes have been synthesized and their spectral characteristics and interaction with double stranded (ds)DNA have been investigated for their prospective use as fluorescent markers in molecular biology. Therefore, the non-covalent binding of the compounds with dsDNA was explored. Apart from the fluorescence spectroscopy, the study includes UV/Vis spectrophotometry and circular dichroism spectroscopy, as well as the thermal melting experiments. Although the compounds show relatively low binding affinitiy towards dsDNA, do not have intrinsic fluorescence, however in the presence of dsDNA they exhibited enhancement in fluorescence intensity. Therefore the studied dyes show interesting platform for future modifications directed towards more sequence selective derivatives. The compound with the highest affinity towards dsDNA showed interesting anti proliferative potential and specificity.

Izvorni jezik
Engleski

Znanstvena područja
Kemija



POVEZANOST RADA


Projekt / tema
098-0982914-2918 - Dizajn, sinteza i ispitivanje interakcija malih molekula s DNA, RNA i proteinima (Ivo Piantanida, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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