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Pregled bibliografske jedinice broj: 5233

Ekspresija retrotranspozona duhana Tto1 u biljci Arabidopsis thaliana L.


Jelenić, Srećko; Bachmair, Andreas
Ekspresija retrotranspozona duhana Tto1 u biljci Arabidopsis thaliana L. // Zbornik sažetaka priopćenja šestog kongresa biologa Hrvatske / Huber, Đ. (ur.).
Zagreb: Hrvatsko biolosko društvo, 1997. (predavanje, nije recenziran, sažetak, znanstveni)


CROSBI ID: 5233 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Ekspresija retrotranspozona duhana Tto1 u biljci Arabidopsis thaliana L.
(Expression of the tobacco retrotransposon Tto1 in the plant Arabidopsis thaliana L.)

Autori
Jelenić, Srećko ; Bachmair, Andreas

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Zbornik sažetaka priopćenja šestog kongresa biologa Hrvatske / Huber, Đ. - Zagreb : Hrvatsko biolosko društvo, 1997

Skup
Šesti Kongres Biologa Hrvatske

Mjesto i datum
Opatija, Hrvatska, 22.-26.09.1997

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
Arabidopsis thaliana L.; retrotranspzon; ekspresija gena
(Arabidopsis thaliana L.; retrotransposons; gene expression)

Sažetak
Retrotransposons are the commonest class of eukaryotic transposable elements and have recently been shown to occupy a large proportion (up to 12%) of all plant genomes. However, little can be said about their contribution (if any) to growth, development and biological fitness of the host organisms. An impediment to investigation of retrotransposons in native host is the considerable background of copies. To circumvent this problem we have tried to express tobacco retrotransposon Tto1 in the plant Arabidopsis thaliana. Heterologous expression of retrotransposons or reconstitution of individual steps of retrotransposon life cycle in heterologous hosts, might be a way to learn more about host-element interaction and might shed light on the interesting problem of horizontal transfer. It would be also very valuable to develop the system for gene tagging based on the retrotransposon particularly for the plant A. thaliana. In this study the roots of A. thaliana were transformed with Tto1 using agrobacterial system. In parallel with wild type, two derivates of Tto1 with mutations in integrase or protease domain have been tested. In order to asses the activity of Tto1 an intron was inserted into the integrase domain in both mutated constructs. In all calli lines derived from roots transformed with wild type Tto1, the homologous recombination between two long terminal repeats of the Tto1 copy in T-DNA has occured. The same has happened with the most protease minus derivates. The rest of the protease minus constructs did not show detectable activity of transposition. In contrary, no homologous recombination occured in integrase minus derivates and all of them underwent reverse transcription as PCR and Southern blot results showed. In this study Tto1 was able to complete its life cycle in heterologous host A. thaliana.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
119112

Ustanove:
Prirodoslovno-matematički fakultet, Zagreb

Profili:

Avatar Url Srećko Jelenić (autor)


Citiraj ovu publikaciju:

Jelenić, Srećko; Bachmair, Andreas
Ekspresija retrotranspozona duhana Tto1 u biljci Arabidopsis thaliana L. // Zbornik sažetaka priopćenja šestog kongresa biologa Hrvatske / Huber, Đ. (ur.).
Zagreb: Hrvatsko biolosko društvo, 1997. (predavanje, nije recenziran, sažetak, znanstveni)
Jelenić, S. & Bachmair, A. (1997) Ekspresija retrotranspozona duhana Tto1 u biljci Arabidopsis thaliana L.. U: Huber, Đ. (ur.)Zbornik sažetaka priopćenja šestog kongresa biologa Hrvatske.
@article{article, author = {Jeleni\'{c}, Sre\'{c}ko and Bachmair, Andreas}, editor = {Huber, \DJ.}, year = {1997}, pages = {70}, keywords = {Arabidopsis thaliana L., retrotranspzon, ekspresija gena}, title = {Ekspresija retrotranspozona duhana Tto1 u biljci Arabidopsis thaliana L.}, keyword = {Arabidopsis thaliana L., retrotranspzon, ekspresija gena}, publisher = {Hrvatsko biolosko dru\v{s}tvo}, publisherplace = {Opatija, Hrvatska} }
@article{article, author = {Jeleni\'{c}, Sre\'{c}ko and Bachmair, Andreas}, editor = {Huber, \DJ.}, year = {1997}, pages = {70}, keywords = {Arabidopsis thaliana L., retrotransposons, gene expression}, title = {Expression of the tobacco retrotransposon Tto1 in the plant Arabidopsis thaliana L.}, keyword = {Arabidopsis thaliana L., retrotransposons, gene expression}, publisher = {Hrvatsko biolosko dru\v{s}tvo}, publisherplace = {Opatija, Hrvatska} }




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