engleski

Factor V Leiden, FII G20210A, PAI-1 (-4G/5G), fibrinogen G(-455)G, FXIII Val34Leu and ACE I/D polymorphism in stroke: a case-control study

Background: Stroke is a multifactorial disease with predisposing genetic and traditional risk factors, such as hypertension, smoking, diabetes and male gender. The aim of this study was to determine whether there is a difference in frequency of these traditional risk factors between stroke patients and control group. Methods: This retrospective study included 250 stroke patients and 100 healthy individuals that served as a control group. Five polymorphisms of the genes included in coagulation were determined: Factor V Leiden and FII G20210A using melting curve analysis ; PA1-1 (-4G/5G), fibrinogen G(-455)A and FXIII Val34Leu using PCR-RFLP method. Also, angiotensin-converting enzyme I/D polymorphism was determined using electrophoretic separation of PCR amplified products. Data on traditional risk factors (gender, hypertension, smoking and diabetes mellitus) were obtained from patient’s medical history. Results: There were no differences in genotype frequencies of any of the tested polymorphism between study groups (all P > 0.05). However, some traditional risk factors were more frequent in the stroke than in control group: diabetes 26.8% vs. 8.1% (P<0.001 ; OR (95% CI) = 3.6 (1.6-8.0)), hypertension 80.8% vs. 59.0% (P<0.001 ; OR (95% CI) = 2.8 (1.6-4.8)) and male gender 52.8% vs. 36.0% (P=0.006 ; OR (95% CI) = 2.3 (1.4-3.9)). Interestingly, there was no statistically significant difference in smoking status (P=0.938). Conclusion: Our results didn’t reveal any association between tested genetic polymorphisms and stroke, while well established traditional factors were identified as significant risk factors. Probably, a larger cohort would detect smaller gene effects of tested polymorphisms.

stroke; polymorphism

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