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Pregled bibliografske jedinice broj: 522016

Recombinant mouse cytomegalovirus expressing a ligand for the NKG2D receptor is attenuated and has improved vaccine properties


Slavuljica, Irena; Busche, Andreas; Babić, Marina; Mitrović, Maja; Gašparović, Iva; Cekinović, Đurđica; Markova Car, Elitza; Pernjak Pugel, Ester; Ciković, Ana; Juranić Lisnić, Vanda et al.
Recombinant mouse cytomegalovirus expressing a ligand for the NKG2D receptor is attenuated and has improved vaccine properties // The Journal of clinical investigation, 120 (2010), 12; 4532-4545 doi:10.1172/JCI43961 (međunarodna recenzija, članak, znanstveni)


Naslov
Recombinant mouse cytomegalovirus expressing a ligand for the NKG2D receptor is attenuated and has improved vaccine properties

Autori
Slavuljica, Irena ; Busche, Andreas ; Babić, Marina ; Mitrović, Maja ; Gašparović, Iva ; Cekinović, Đurđica ; Markova Car, Elitza ; Pernjak Pugel, Ester ; Ciković, Ana ; Juranić Lisnić, Vanda ; Britt, William J. ; Koszinowski, Ulrich ; Messerle, Martin ; Krmpotić, Astrid ; Jonjić, Stipan

Izvornik
The Journal of clinical investigation (0021-9738) 120 (2010), 12; 4532-4545

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
MCMV; NKG2D; CMV vaccine

Sažetak
Human CMV (HCMV) is a major cause of morbidity and mortality in both congenitally infected and immunocompromised individuals. Development of an effective HCMV vaccine would help protect these vulnerable groups. NK group 2, member D (NKG2D) is a potent activating receptor expressed by cells of the innate and adaptive immune systems. Its importance in HCMV immune surveillance is indicated by the elaborative evasion mechanisms evolved by the virus to avoid NKG2D. In order to study this signaling pathway, we engineered a recombinant mouse CMV expressing the high-affinity NKG2D ligand RAE-1γ (RAE-1γMCMV). Expression of RAE-1γ by MCMV resulted in profound virus attenuation in vivo and lower latent viral DNA loads. RAE-1γMCMV infection was efficiently controlled by immunodeficient hosts, including mice lacking type I interferon receptors or immunosuppressed by sublethal γ-irradiation. Features of MCMV infection in neonates were also diminished. Despite tight innate immune control, RAE-1γMCMV infection elicited strong and long-lasting protective immunity. Maternal RAE-1γMCMV immunization protected neonatal mice from MCMV disease via placental transfer of antiviral Abs. Despite strong selective pressure, the RAE-1γ transgene did not exhibit sequence variation following infection. Together, our results indicate that use of a recombinant virus encoding the ligand for an activating NK cell receptor could be a powerful approach to developing a safe and immunogenic HCMV vaccine.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
062-0621261-1263 - Molekularni mehanizmi citomegalovirusnog izmicanja imunološkom nadzoru (Stipan Jonjić, )
062-0621261-1268 - Uloga imunosubverzivnih citomegalovirusnih gena u latenciji (Astrid Krmpotić, )
062-0621261-1269 - Perinatalni citomegalovirusni encefalitis (Ester Pernjak-Pugel, )

Ustanove
Medicinski fakultet, Rijeka

Citiraj ovu publikaciju

Slavuljica, Irena; Busche, Andreas; Babić, Marina; Mitrović, Maja; Gašparović, Iva; Cekinović, Đurđica; Markova Car, Elitza; Pernjak Pugel, Ester; Ciković, Ana; Juranić Lisnić, Vanda et al.
Recombinant mouse cytomegalovirus expressing a ligand for the NKG2D receptor is attenuated and has improved vaccine properties // The Journal of clinical investigation, 120 (2010), 12; 4532-4545 doi:10.1172/JCI43961 (međunarodna recenzija, članak, znanstveni)
Slavuljica, I., Busche, A., Babić, M., Mitrović, M., Gašparović, I., Cekinović, Đ., Markova Car, E., Pernjak Pugel, E., Ciković, A. & Juranić Lisnić, V. (2010) Recombinant mouse cytomegalovirus expressing a ligand for the NKG2D receptor is attenuated and has improved vaccine properties. The Journal of clinical investigation, 120 (12), 4532-4545 doi:10.1172/JCI43961.
@article{article, year = {2010}, pages = {4532-4545}, DOI = {10.1172/JCI43961}, keywords = {MCMV, NKG2D, CMV vaccine}, journal = {The Journal of clinical investigation}, doi = {10.1172/JCI43961}, volume = {120}, number = {12}, issn = {0021-9738}, title = {Recombinant mouse cytomegalovirus expressing a ligand for the NKG2D receptor is attenuated and has improved vaccine properties}, keyword = {MCMV, NKG2D, CMV vaccine} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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