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Evidence for taxonomic and functional drift of an atrazine-degrading culture in response to high atrazine input


Udiković-Kolić, Nikolina; Devers-Lamrani, Marion; Petrić, Ines; Hršak, Dubravka; Martin-Laurent, Fabrice
Evidence for taxonomic and functional drift of an atrazine-degrading culture in response to high atrazine input // Applied microbiology and biotechnology, 90 (2011), 4; 1547-1554 doi:10.1007/s00253-011-3198-2 (međunarodna recenzija, članak, znanstveni)


Naslov
Evidence for taxonomic and functional drift of an atrazine-degrading culture in response to high atrazine input

Autori
Udiković-Kolić, Nikolina ; Devers-Lamrani, Marion ; Petrić, Ines ; Hršak, Dubravka ; Martin-Laurent, Fabrice

Izvornik
Applied microbiology and biotechnology (0175-7598) 90 (2011), 4; 1547-1554

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Atrazine; degradation; bacterial culture; evolution; atz genes; trz genes

Sažetak
We evaluated the effects of variations in atrazine input on the evolution of a bacterial culture adapted to a low atrazine concentration. This initial culture (M3-K) was subjected to weekly subculturing in the presence of a high concentration of atrazine as the only N source (100 mg l−1). After four subculturing, M3-K evolved to a new bacterial culture (M3) which exhibited a significant increase in the extent of atrazine mineralization in comparison with the initial culture. Molecular analyses of M3-K and M3 cultures by cloning, restriction analysis, and sequencing of the 16S rRNA genes revealed significant differences in culture structure and composition. M3-K culture comprised mainly Actinobacteria (40%), β-Proteobacteria (26%), and Bacteroidetes (16%). After exposure to a high atrazine concentration, the dominance of Actinobacteria decreased (14%), Bacteroidetes increased (27%), and β-Proteobacteria were replaced by γ-Proteobacteria (32%). Quantitative PCR revealed that the abundance of atzB and atzC genes relative to total bacteria decreased by a factor of 3–4 following the increase in atrazine concentration, while the relative abundance of trzD increased significantly (≈400 times). Presented study shows that variations in atrazine input drive both functional and compositional shifts in the atrazine-degrading bacterial culture.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija



POVEZANOST RADA


Projekt / tema
098-0982934-2712 - Organski spojevi kao molekulski obilježivači antropogenog utjecaja na okoliš (Marijan Ahel, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus


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