Evidence for taxonomic and functional drift of an atrazine-degrading culture in response to high atrazine input (CROSBI ID 173160)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Udiković-Kolić, Nikolina ; Devers-Lamrani, Marion ; Petrić, Ines ; Hršak, Dubravka ; Martin-Laurent, Fabrice
engleski
Evidence for taxonomic and functional drift of an atrazine-degrading culture in response to high atrazine input
We evaluated the effects of variations in atrazine input on the evolution of a bacterial culture adapted to a low atrazine concentration. This initial culture (M3-K) was subjected to weekly subculturing in the presence of a high concentration of atrazine as the only N source (100 mg l−1). After four subculturing, M3-K evolved to a new bacterial culture (M3) which exhibited a significant increase in the extent of atrazine mineralization in comparison with the initial culture. Molecular analyses of M3-K and M3 cultures by cloning, restriction analysis, and sequencing of the 16S rRNA genes revealed significant differences in culture structure and composition. M3-K culture comprised mainly Actinobacteria (40%), β-Proteobacteria (26%), and Bacteroidetes (16%). After exposure to a high atrazine concentration, the dominance of Actinobacteria decreased (14%), Bacteroidetes increased (27%), and β-Proteobacteria were replaced by γ-Proteobacteria (32%). Quantitative PCR revealed that the abundance of atzB and atzC genes relative to total bacteria decreased by a factor of 3–4 following the increase in atrazine concentration, while the relative abundance of trzD increased significantly (≈400 times). Presented study shows that variations in atrazine input drive both functional and compositional shifts in the atrazine-degrading bacterial culture.
atrazine; degradation; bacterial culture; evolution; atz genes; trz genes
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nije evidentirano
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Podaci o izdanju
90 (4)
2011.
1547-1554
objavljeno
0175-7598
10.1007/s00253-011-3198-2