engleski

Influence of freeze-drying and microencapsulation on functionality of probiotic strains in the intestinal tract

In order to exert the probiotic activity in the human intestinal tract, probiotic preparations need to contain 106-108 viable cells per gram of the product. Therefore, freeze-drying of the wet cell biomass, with the different lyoprotectors was performed, with the aim to produce probiotic strains Lactobacillus helveticus M92, Lactobacillus plantarum L4 and Enterococcus faecium L3 in the powder form with the high viable cell count for the application as biotherapeutics. Skim milk and inulin have shown as optimal lyoprotectors between tested ones, as higher viability of probiotic cells was achieved after the freeze-drying compared to control and other lyoprotectors. The viable cell count of probiotic strains, after freeze-drying in skim milk, was above 107 CFU/g, during 1 year of storage at 4º C what is in accordance with the claims set up for the probiotic products. Another approach for increasing the viable cell number of probiotic strains, during freeze-drying and storage as well as for an oral target delivery, could be microencapsulation. Hence, the microencapsulation of wet biomass and freeze-dried bacteria cells of L. helveticus M92, L. plantarum L4 and E. faecium L3 were evaluated. The microencapsulation of probiotic cells was performed in alginate, ĸ -carrageenan and by transglutaminase-induced gelatination of caseinate in order to investigate the effect of the microencapsulation on the functionality of probiotic strains. The higher percentage of the survival of microencapsulated cells of L. helveticus M92, L. plantarum L4 and E. faecium L3 compared to the percentage of the survival of non-microencapsulated probiotic cells, in simulated gastrointestinal tract conditions, indicates the protective effect of the microencapsulation on the probiotic strains.

freeze-drying; microencapsulation; oral delivery; probiotics; simulated gastrointestinal tract conditions

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