engleski

Croatia launches national referent Y-STR haplotype database

Creation of a national referent Y-chromosome short tandem repeat (STR) haplotype database is one of the principal steps in a detailed genetic characterization. The aim of this study was to create a comprehensive Y-STR haplotype database of the Croatian contemporary population and to analyze substructure between the five Croatian regions. We carried out a statistical analysis of the data from previously performed genetic analyses collected during routine forensic work by the Forensic Science Centre “Ivan Vučetić. A total of 1100 unrelated men from eastern, western, northern, southern and central Croatia were included in this study. Genomic DNA was extracted from FTA® cards using Chelex. 17 Y-STR loci were amplified using the AmpFISTR Yfiler PCR amplification kit. The haplotype frequencies were determined by direct counting and analyzed using Arlequin 3.1. AMOVA analysis calculated with the YHRD online analysis tool included 16 population samples with 19926 haplotypes. A total of 947 haplotypes were recorded, 848 of which were unique. Haplotype diversity was 0.998, with the common recorded haplotype found in 9 of 1100 men. Locus diversity varied from 0.267 for DYS392 to 0.772 for DYS458. Discrimination capacity was 86.1%. The results suggest high level of similarity among regional sub-populations within Croatia, and high level of resemblance with some south-eastern population groups from Bosnia and Herzegovina and rather strongly expressed difference from the north-western neighboring countries. The database will serve not only as a source of relevant information for forensic purposes, but also as a source of useful information in population genetics.

Croatia; national referent; Y-STR haplotype; database

Presentation No FG 16 ; Abstr. No. ABS-109-ISABS-2011