Use of UV photography to identify aflatoxin-producing strains of Aspergillus flavus and A. parasiticus (CROSBI ID 89104)
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Cvetnić, Zdenka ; Pepeljnjak, Stjepan
engleski
Use of UV photography to identify aflatoxin-producing strains of Aspergillus flavus and A. parasiticus
UV photography in glucose, yeast exstract (GY) agar medium was tested as a simple and rapid method for the distinction of aflatoxin-positive from aflatoxin-negative strains of Aspergillus flavus and A. parasiticus. In the UV photographs aflatoxin producing moulds were identified as grey or black colonies, wheras aflatoxin non-producing moulds appeared as white colonies. Of the aflatoxin positive starins detected by UV photographic method, 10 % was confirmed by extraction of the GY agar medium and mould mycelium in chloroform extracts, which were analysed subsequently using thin layer chromatography. Confirmation of aflatoxigenic strains was achieved by biosynthesis on liquid medium yeast extract sucrose (YES) broth.
UV photography; aflatoxin; Aspergillus flavus; Aspergillus parasiticus
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