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Abrupt Telomere Shortening and the stochastic nature of cellular aging (CROSBI ID 573819)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa | međunarodna recenzija

Ivanković, Milena ; Škrobot Vidaček, Nikolina ; Ćukušić Kalajžić, Andrea ; Rubelj, Ivica Abrupt Telomere Shortening and the stochastic nature of cellular aging // Telomeres and Telomerase / de Lange, Titia ; Reddel, Roger ; Shippen, Dorothy et al. (ur.). Lahti: Cold Spring Harbor Laboratory (CSHL), 2011. str. 204-204

Podaci o odgovornosti

Ivanković, Milena ; Škrobot Vidaček, Nikolina ; Ćukušić Kalajžić, Andrea ; Rubelj, Ivica

engleski

Abrupt Telomere Shortening and the stochastic nature of cellular aging

We can say that telomeres are among the most popular biological structures today! Their involvement in some of the most important biological phenomena such as aging and cancer is widely recognized. They are the physical ends of all eukaryotic chromosomes, protecting their integrity and consequently maintaining genome stability. They are evolutionarily conserved so that all vertebrates share the same repetitive sequence (TTAGGG)n. Telomeres are dynamic structures, in normal somatic cells they gradually shorten with each cell division which ultimately leads to cell senescence. Experimental evidence suggests that accumulation of senescent cells in tissues contributes to aging and development of age related diseases. Telomere length is a reliable marker for longevity, healthspan, occurrence of cancer and various other diseases in the population. Therefore, there are many reasons to engage in research on telomere biology. We have employed a novel methodology to provide evidence for the generation of extra chromosomal circular telomeric DNA as a result of abrupt telomere shortening in normal human fibroblasts. Also, using original techniques we examined the behavior of individual telomeres. We identified unique subtelomeric regions, which are large enough to produce detectable chemiluminescent signals. This enabled us to follow individual Xp/Yp telomeres by Southern blot analysis and to test the model of abrupt telomere shortening as well as to compare these results with quantitative PNA-FISH analysis. Q-PNA-FISH analysis of individual Y chromosome demonstrated unequal shortening dynamics between its p and q telomeres. We believe that abrupt telomere shortening is of great importance for our understanding of the mechanistic basis of aging because it ensures heterogeneity in growth potential among individual cells, which is crucial for the gradual progression of the aging process at the organismal level.

Abrupt Telomere Shortening; Stochastic Cellular Aging

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Podaci o prilogu

204-204.

2011.

objavljeno

Podaci o matičnoj publikaciji

Telomeres and Telomerase

de Lange, Titia ; Reddel, Roger ; Shippen, Dorothy ; Zakian, Virginia

Lahti: Cold Spring Harbor Laboratory (CSHL)

Podaci o skupu

Telomeres and Telomerase

predavanje

03.05.2011-07.05.2011

Cold Spring Harbor (NY), Sjedinjene Američke Države

Povezanost rada

Biologija