engleski

PTCH gene altered in dentigerous cysts

Motivated by the evidence that odontogenic keratocysts are associated with genetic alterations, we have been examining the possibility that development of other odontogenic cysts can be attributed to gene malfunctioning, in particular to the PTCH gene. Cyst epithelium has been examined for polymorphism on chromosome 9q22.3, the region that contains the PTCH. Loss of heterozygosity (LOH) for D9S287 and/or D9S180 marker was observed in about 50 % of dentigerous cysts, whereas radicular cysts gave no indication of lesions in the PTCH region. As a more direct argument for PTCH involvement in cyst growth, we report evidence of PTCH expression in dentigerous syst lining, which indicates malfunctioning of the relevant signaling pathway. While we found no reason to believe that PTCH should be associated with radicular cysts, other genes may perhaps be implicated in their development. We have performed immunohistochemical comparison of keratocysts, dentigerous and radicular cysts for nonmetastatic marker Nm23.Graded response placed radicular cysts between the other two types, suggesting a similar neoplastic character of their epithelial proliferation.

tumor suppressor; PTCH; patched; dentigerous cystss; keratocysts; radicular cysts; Nm23

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