First evidence for the presence of efflux pump in the earthworm Eisenia andrei (CROSBI ID 172535)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Hackenberger Kutuzović, Branimir ; Velki, Mirna ; Stepić, Sandra ; Hackenberger Kutuzović, Davorka
engleski
First evidence for the presence of efflux pump in the earthworm Eisenia andrei
Efflux pumps are transport proteins involved in the extrusion of toxic substrates from cells to the external environment. Activities of efflux pumps have been found in many organisms, however such activity has not been evidenced in earthworms. Adult Eisenia andrei earthworms were exposed to efflux modulators – verapamil (a known inhibitor of efflux pump protein) and dexamethasone (a known inducer of efflux activity) – and the amount of absorbed fluorescent dye rhodamine B was measured. The results showed that verapamil inhibited efflux activity and decreased removal of rhodamine B, whereas dexamethasone induced efflux activity and increased removal of rhodamine B. This is the first evidence of the presence of efflux pump in earthworm Eisenia andrei. Since earthworms are often used as test organisms due to their sensitive reactions towards environmental influences, the discovery of efflux pump activity can contribute to the better understanding of toxicity of certain pollutants.
efflux activity; verapamil; dexamethasone; rhodamine B; biomarker
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nije evidentirano
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Podaci o izdanju
75
2012.
40-45
objavljeno
0147-6513
10.1016/j.ecoenv.2011.06.024
Povezanost rada
Biologija