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izvor podataka: crosbi

Differentiation of Croatian barley varieties by gradient gel SDS-PAGE and isoelectric focusing of dry grains and green malt hordeins (CROSBI ID 172472)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Strelec, Ivica ; Has-Schön, Elizabeta ; Vitale, Ljubinka Differentiation of Croatian barley varieties by gradient gel SDS-PAGE and isoelectric focusing of dry grains and green malt hordeins // Poljoprivreda (Osijek), 17 (2011), 1; 23-27

Podaci o odgovornosti

Strelec, Ivica ; Has-Schön, Elizabeta ; Vitale, Ljubinka

engleski

Differentiation of Croatian barley varieties by gradient gel SDS-PAGE and isoelectric focusing of dry grains and green malt hordeins

Applicability of polyacrylamide gel electrophoresis and isoelectric focusing of hordeins for discrimination of six two-rowed winter barley varieties (Angora, Sladoran, Rodnik, Rex, Martin and Barun) has been investigated. Hordeins extracted from dry grains and green malt of barley varieties were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis in gradient gel of 8-18% T, and by isoelectric focusing in pH gradient of 3.5-9.5 and 5.5-8.5, respectively. In all separation experiments better resolution of proteins was achieved with dry grain extracts, than with malt extracts. Angora, Sladoran and Martin variety could be distinguished from other varieties by differences in hordein patterns obtained by gradient gel SDS-PAGE (8-18% T), and Angora, Sladoran, Martin and Rodnik by isoelectric focusing in pH gradient 5.5-8.5.

barley variety discrimination ; gradient gel SDS-PAGE ; hordeins ; isoelectric focusing

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Podaci o izdanju

17 (1)

2011.

23-27

objavljeno

1330-7142

1848-8080

Povezanost rada

Biotehnologija, Prehrambena tehnologija

Poveznice
Indeksiranost