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Pregled bibliografske jedinice broj: 517162

Differentiation of Croatian barley varieties by gradient gel SDS-PAGE and isoelectric focusing of dry grains and green malt hordeins


Strelec, Ivica; Has-Schön, Elizabeta; Vitale, Ljubinka
Differentiation of Croatian barley varieties by gradient gel SDS-PAGE and isoelectric focusing of dry grains and green malt hordeins // Poljoprivreda (Osijek), 17 (2011), 1; 23-27 (međunarodna recenzija, članak, znanstveni)


Naslov
Differentiation of Croatian barley varieties by gradient gel SDS-PAGE and isoelectric focusing of dry grains and green malt hordeins

Autori
Strelec, Ivica ; Has-Schön, Elizabeta ; Vitale, Ljubinka

Izvornik
Poljoprivreda (Osijek) (1330-7142) 17 (2011), 1; 23-27

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Barley variety discrimination ; gradient gel SDS-PAGE ; hordeins ; isoelectric focusing

Sažetak
Applicability of polyacrylamide gel electrophoresis and isoelectric focusing of hordeins for discrimination of six two-rowed winter barley varieties (Angora, Sladoran, Rodnik, Rex, Martin and Barun) has been investigated. Hordeins extracted from dry grains and green malt of barley varieties were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis in gradient gel of 8-18% T, and by isoelectric focusing in pH gradient of 3.5-9.5 and 5.5-8.5, respectively. In all separation experiments better resolution of proteins was achieved with dry grain extracts, than with malt extracts. Angora, Sladoran and Martin variety could be distinguished from other varieties by differences in hordein patterns obtained by gradient gel SDS-PAGE (8-18% T), and Angora, Sladoran, Martin and Rodnik by isoelectric focusing in pH gradient 5.5-8.5.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija, Prehrambena tehnologija



POVEZANOST RADA


Projekt / tema
113-1130473-0334 - Sinergističke smjese u antifungalnoj i antimikotoksigenoj zaštiti hrane (Tomislav Klapec, )
113-1130475-2722 - Unapređenje kakvoće, trajnosti i funkcionalnosti proizvoda na bazi pšenice (Daliborka Koceva Komlenić, )

Ustanove
Institut "Ruđer Bošković", Zagreb,
Prehrambeno-tehnološki fakultet, Osijek,
Sveučilište u Osijeku - Odjel za biologiju

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Emerging Sources Citation Index (ESCI)
  • Scopus


Uključenost u ostale bibliografske baze podataka:


  • AGRICOLA
  • CAB Abstracts