engleski

Carbendazim combned with imazalil or cypermethrin potentiate DNA damage in hepatocytes of mice

Traces of pesticides imazalil, cypermethrin and carbendazim are detected in plants for human consumption. To explore if their orally applied combinations will induce DNA breaks in hepatocytes, a subchronic in vivo experiment was performed in Swiss mice. Doses of 10 mg kg-1 of imazalil (im) and cypermethrin (cy) and 20 mg kg-1 of carbendazim (car) and their combinations (im, 10 mg kg- 1+cy, 10 mg kg-1 ; im, 10 mg kg-1+car, 20 mgkg-1 ; car 20 mg kg- 1+ cy, 10 mg kg-1) were applied daily during 28 days. Afterwards DNA damage in hepatocytes was evaluated by comet assay. Individually imazalil and cypermethrin damaged DNA at alkali labile sites while TM of carbendazim alone was similar to control but with higher tail length. In combination with carbendazim clastogen properties of imazalils and cypermethrins were potentiated compared to all other treatments and control. There were pronounced sex differences in pattern of fragmentation between treated groups. Higher LTN in females indicates that in females certain cells were especially prone to total nucleus disintegration. Due to synergistic effects low environmentally present concentrations of imazalil and cypermethrin in food, and especially their mixtures with carbendazim have genotoxic potential that could be particularly dangerous over prolonged exposure in mammalian organism.

DNA repair; Human toxicology; Pesticide toxicology; Hepatic

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