engleski

Preliminary Observations on the Phytosanitary Status of the Croatian Satsuma Mandarin (Citrus unshiu Marc.) Collection

The presence of graft-transmissible pathogens in Croatia, especially Citrus tristeza virus (CTV) had been previously confirmed in citrus field trees during preliminary surveys. The highest occurrence of CTV was recorded in satsuma mandarin (Citrus unshiu Marc.), commercially the most important citrus in the country. With the aim of assessing the phytosanitary status of the established satsuma collection, biological indexing of graft-transmissible pathogens was performed, followed by serological assay of CTV and Citrus psorosis virus (CPsV). Results of 18 tested clones from the collection orchard showed that only two clones were CTV-negative, while they were all CPsV-negative, both in biological and laboratory tests. Furthermore, severe CTV strains displaying stem pitting and seedling yellows in grapefruit and sweet oranges were found in 4 tested clones. No biological indication of other disease presence in the collection has been found so far. Further testing of the satsuma collection is underway, as well as the sanitation of the most important cultivars.

graft-transmissible pathogens; ELISA; DTBIA; biological indexing

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