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Pregled bibliografske jedinice broj: 514183

Manning free counterion fraction for a rodlike polyion: Aqueous solutions of short DNA fragments in presence of very low added salt


Vuletić, Tomislav; Dolanski Babić, Sanja; Grgičin, Danijel; Aumiler, Damir; Raedler, Joachim; Livolant, Francoise; Tomić, Silvia
Manning free counterion fraction for a rodlike polyion: Aqueous solutions of short DNA fragments in presence of very low added salt // Physical Review E - Statistical, Nonlinear, and Soft Matter Physics, 83 (2011), 4; 041803-1 doi:10.1103/PhysRevE.83.041803 (međunarodna recenzija, članak, znanstveni)


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Naslov
Manning free counterion fraction for a rodlike polyion: Aqueous solutions of short DNA fragments in presence of very low added salt

Autori
Vuletić, Tomislav ; Dolanski Babić, Sanja ; Grgičin, Danijel ; Aumiler, Damir ; Raedler, Joachim ; Livolant, Francoise ; Tomić, Silvia

Izvornik
Physical Review E - Statistical, Nonlinear, and Soft Matter Physics (1539-3755) 83 (2011), 4; 041803-1

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Manning condensation ; rodlike polyelectrolyte ; DNA ; FCS ; conductometry

Sažetak
We quantified the Manning free (uncondensed) counterions fraction $\theta$ for dilute aqueous solutions of rod-like polyions - 150bp DNA fragments, in presence of very low concentration of monovalent salt $c_{; ; \mathrm{; ; salt}; ; }; ; <0.05$mM. Conductivity measurements of these solutions for DNA basepair concentration range $0.015\leq c \leq 8$~mM were complemented by fluorescence correlation spectroscopy (FCS) measurements of the DNA polyion diffusion coefficient $D_p(c)$. We observed a crossover in the normalized conductivity $\sigma(c)/c$ which nearly halved across $c=0.05-1$ mM range, while $D_p(c)$ remained rather constant, as we established by FCS. Analyzing these data we extracted $\theta(c)=0.30 - 0.45$, and taking the Manning asymmetry field effect on polyelectrolyte conductivity into account we got $\theta(c)=0.40-0.60$. We relate the $\theta(c)$ variation to gradual DNA denaturation occuring, in the very low salt environment, with the decrease in DNA concentration itself. The extremes of the experimental $\theta(c)$ range occur towards the highest, above 1 mM and the lowest, below 0.05 mM, DNA concentrations, and correspond to the theoretical $\theta$ values for dsDNA and ssDNA, respectively. Therefore, we confirmed Manning condensation and conductivity models to be valuable in description of dilute solutions of rod-like polyions.

Izvorni jezik
Engleski

Znanstvena područja
Fizika



POVEZANOST RADA


Projekti:
035-0000000-2836 - Jako korelirani anorganski, organski i biomaterijali (Tomić, Silvia, MZOS ) ( POIROT)

Ustanove:
Institut za fiziku, Zagreb

Poveznice na cjeloviti tekst rada:

Pristup cjelovitom tekstu rada doi link.aps.org

Citiraj ovu publikaciju:

Vuletić, Tomislav; Dolanski Babić, Sanja; Grgičin, Danijel; Aumiler, Damir; Raedler, Joachim; Livolant, Francoise; Tomić, Silvia
Manning free counterion fraction for a rodlike polyion: Aqueous solutions of short DNA fragments in presence of very low added salt // Physical Review E - Statistical, Nonlinear, and Soft Matter Physics, 83 (2011), 4; 041803-1 doi:10.1103/PhysRevE.83.041803 (međunarodna recenzija, članak, znanstveni)
Vuletić, T., Dolanski Babić, S., Grgičin, D., Aumiler, D., Raedler, J., Livolant, F. & Tomić, S. (2011) Manning free counterion fraction for a rodlike polyion: Aqueous solutions of short DNA fragments in presence of very low added salt. Physical Review E - Statistical, Nonlinear, and Soft Matter Physics, 83 (4), 041803-1 doi:10.1103/PhysRevE.83.041803.
@article{article, author = {Vuleti\'{c}, Tomislav and Dolanski Babi\'{c}, Sanja and Grgi\v{c}in, Danijel and Aumiler, Damir and Raedler, Joachim and Livolant, Francoise and Tomi\'{c}, Silvia}, year = {2011}, pages = {041803-1-041803-8}, DOI = {10.1103/PhysRevE.83.041803}, keywords = {Manning condensation, rodlike polyelectrolyte, DNA, FCS, conductometry}, journal = {Physical Review E - Statistical, Nonlinear, and Soft Matter Physics}, doi = {10.1103/PhysRevE.83.041803}, volume = {83}, number = {4}, issn = {1539-3755}, title = {Manning free counterion fraction for a rodlike polyion: Aqueous solutions of short DNA fragments in presence of very low added salt}, keyword = {Manning condensation, rodlike polyelectrolyte, DNA, FCS, conductometry} }
@article{article, author = {Vuleti\'{c}, Tomislav and Dolanski Babi\'{c}, Sanja and Grgi\v{c}in, Danijel and Aumiler, Damir and Raedler, Joachim and Livolant, Francoise and Tomi\'{c}, Silvia}, year = {2011}, pages = {041803-1-041803-8}, DOI = {10.1103/PhysRevE.83.041803}, keywords = {Manning condensation, rodlike polyelectrolyte, DNA, FCS, conductometry}, journal = {Physical Review E - Statistical, Nonlinear, and Soft Matter Physics}, doi = {10.1103/PhysRevE.83.041803}, volume = {83}, number = {4}, issn = {1539-3755}, title = {Manning free counterion fraction for a rodlike polyion: Aqueous solutions of short DNA fragments in presence of very low added salt}, keyword = {Manning condensation, rodlike polyelectrolyte, DNA, FCS, conductometry} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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