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Pregled bibliografske jedinice broj: 514050

Chemical composition of the ethanolic propolis extracts and its effect on HeLa cells


Barbarić, Monika; Mišković, Katarina; Bojić, Mirza; Baus Lončar, Mirela; Smolčić-Bubalo, Asja; Debeljak, Željko; Medić-Šarić, Marica
Chemical composition of the ethanolic propolis extracts and its effect on HeLa cells // Journal of ethnopharmacology, 135 (2011), 3; 772-778 doi:10.1016/j.jep.2011.04.015 (međunarodna recenzija, članak, znanstveni)


Naslov
Chemical composition of the ethanolic propolis extracts and its effect on HeLa cells

Autori
Barbarić, Monika ; Mišković, Katarina ; Bojić, Mirza ; Baus Lončar, Mirela ; Smolčić-Bubalo, Asja ; Debeljak, Željko ; Medić-Šarić, Marica

Izvornik
Journal of ethnopharmacology (0378-8741) 135 (2011), 3; 772-778

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Propolis ; HPLC ; phenolic acids ; flavonoids ; HeLa ; cytotoxic evaluation

Sažetak
Ethnopharmacological relevance Propolis is a resinous hive product collected by honeybees from various plant sources. It is widely used in traditional medicine and is reported to have a broad spectrum of pharmacological effects (antibacterial, antihepatoxic, antioxidative, anti-inflammatory, etc.). Thus the aim of this study was to assess cytotoxic effect of various ethanol propolis extractions on the cervical tumor cell line (HeLa) and compare it with their phenolic acids and flavonoids composition. Materials and methods Twenty samples of raw propolis were collected from 17 localities of Croatia (I–XVII), 2 of Bosnia and Hercegovina (XVIII, XIX) and 1 of Macedonia (XX). Reverse phase HPLC was used to determine the chemical composition of polyphenols. Biological experiments were carried out in vitro on cervix adenocarcinoma cell line (HeLa). Results Phenolic acids (ferulic acid, p-coumaric acid, caffeic acid) and flavonoids (tectochrysin, galangin, pinocembrin, pinocembrin-7-methylether, chrysin, apigenin, kaempferol, quercetin) have been determined using HPLC analysis in 20 ethanolic propolis extracts. All samples contain tectochrysin in ranges of 0.1988 mg/g (XVIII) to 1.2004 mg/g (III), while caffeic acid and quercetin have not been found. Flavonoid that is most abundant is galangin in ranges from 0.3706 mg/g (XVII) to 47.4879 mg/g (IX). The samples of propolis numbers I, VI and X applied in the investigated concentration range manifested significant reduction of cell growth. GI50 value as indicator of cytotoxicity among propolis samples showed that propolis number VII is the most effective (GI50 = 76 μg/ml) followed by propolis nos. XV, XVIII and I. Conclusion Antiproliferative and cytotoxic effect of propolis on the HeLa cells is not correlating with the concentration of particular components but on establishing the possible synergistic antiproliferative activity of individual phenolic acid and flavonoids. Differences in the chemical composition lead to diversity in biological activity of propolis samples.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Temeljne medicinske znanosti, Farmacija



POVEZANOST RADA


Projekt / tema
006-0061117-1237 - Biološki aktivni spojevi, metaboliti i QSAR (Marica Medić-Šarić, )
219-0982914-2176 - Mehanizam bioloških učinaka novih malih molekula na stanice tumora čovjeka (Ljubica Glavaš Obrovac, )
219-0982914-2179 - Uloga malih zaštitinih TFF proteina u zdravlju i bolesti (Tatjana Belovari, )

Ustanove
Farmaceutsko-biokemijski fakultet, Zagreb,
Institut "Ruđer Bošković", Zagreb,
Medicinski fakultet, Osijek

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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