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Pregled bibliografske jedinice broj: 511940

Inter-laboratory evaluation of the ISO standard 11063 "Soil quality - Method to directly extract DNA from soil samples"


Petrić, Ines; Philippot, L.; Abbate, C.; Bispo, A.; Chesnot, T.; Hallin, S.; Laval, K.; Lebeau, T.; Lemanceau, P.; Leyval, C. et al.
Inter-laboratory evaluation of the ISO standard 11063 "Soil quality - Method to directly extract DNA from soil samples" // Journal of microbiological methods, 84 (2011), 3; 454-460 doi:10.1016/j.mimet.2011.01.016 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 511940 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Inter-laboratory evaluation of the ISO standard 11063 "Soil quality - Method to directly extract DNA from soil samples"

Autori
Petrić, Ines ; Philippot, L. ; Abbate, C. ; Bispo, A. ; Chesnot, T. ; Hallin, S. ; Laval, K. ; Lebeau, T. ; Lemanceau, P. ; Leyval, C. ; Lindström, K. ; Pandard, P. ; Romero, E. ; Sarr, A. ; Schloter, M. ; Simonet, P. ; Smalla, K. ; Wilke, B.M. ; Martin-Laurent, F.

Izvornik
Journal of microbiological methods (0167-7012) 84 (2011), 3; 454-460

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
soil DNA extraction ; standardization ; inter-laboratory assay ; DNA fingerprint ; qPCR

Sažetak
Extracting DNA directly from micro-organisms living in soil is a crucial step for the molecular analysis of soil microbial communities. However, the use of a plethora of different soil DNA extraction protocols, each with its own bias, makes accurate data comparison difficult. To overcome this problem, a method for soil DNA extraction was proposed to the International Organization for Standardization (ISO) in 2006. This method was evaluated by 13 independent European laboratories actively participating in national and international ring tests. The reproducibility of the standardized method for molecular analyses was evaluated by comparing the amount of DNA extracted, as well as the abundance and genetic structure of the total bacterial community in the DNA extracted from 12 different soils by the 13 laboratories. High quality DNA was successfully extracted from all 12 soils, despite different physical and chemical characteristics and a range of origins from arable soils, through forests to industrial sites. Quantification of the 16S rRNA gene abundances by real time PCR and analysis of the total bacterial community structure by automated ribosomal intergenic spacer analysis (A-RISA) showed acceptable to good levels of reproducibility. Based on the results of both ring-tests, the method was unanimously approved by the ISO as an international standard method and the normative protocol will now be disseminated within the scientific community. Standardization of a soil DNA extraction method will improve data comparison, facilitating our understanding of soil microbial diversity and soil quality monitoring.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
098-0982934-2712 - Organski spojevi kao molekulski obilježivači antropogenog utjecaja na okoliš (Ahel, Marijan, MZOS ) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Ines Sviličić Petrić (autor)

Poveznice na cjeloviti tekst rada:

doi dx.doi.org www.sciencedirect.com

Citiraj ovu publikaciju:

Petrić, Ines; Philippot, L.; Abbate, C.; Bispo, A.; Chesnot, T.; Hallin, S.; Laval, K.; Lebeau, T.; Lemanceau, P.; Leyval, C. et al.
Inter-laboratory evaluation of the ISO standard 11063 "Soil quality - Method to directly extract DNA from soil samples" // Journal of microbiological methods, 84 (2011), 3; 454-460 doi:10.1016/j.mimet.2011.01.016 (međunarodna recenzija, članak, znanstveni)
Petrić, I., Philippot, L., Abbate, C., Bispo, A., Chesnot, T., Hallin, S., Laval, K., Lebeau, T., Lemanceau, P. & Leyval, C. (2011) Inter-laboratory evaluation of the ISO standard 11063 "Soil quality - Method to directly extract DNA from soil samples". Journal of microbiological methods, 84 (3), 454-460 doi:10.1016/j.mimet.2011.01.016.
@article{article, author = {Petri\'{c}, Ines and Philippot, L. and Abbate, C. and Bispo, A. and Chesnot, T. and Hallin, S. and Laval, K. and Lebeau, T. and Lemanceau, P. and Leyval, C. and Lindstr\"{o}m, K. and Pandard, P. and Romero, E. and Sarr, A. and Schloter, M. and Simonet, P. and Smalla, K. and Wilke, B.M. and Martin-Laurent, F.}, year = {2011}, pages = {454-460}, DOI = {10.1016/j.mimet.2011.01.016}, keywords = {soil DNA extraction, standardization, inter-laboratory assay, DNA fingerprint, qPCR}, journal = {Journal of microbiological methods}, doi = {10.1016/j.mimet.2011.01.016}, volume = {84}, number = {3}, issn = {0167-7012}, title = {Inter-laboratory evaluation of the ISO standard 11063 "Soil quality - Method to directly extract DNA from soil samples"}, keyword = {soil DNA extraction, standardization, inter-laboratory assay, DNA fingerprint, qPCR} }
@article{article, author = {Petri\'{c}, Ines and Philippot, L. and Abbate, C. and Bispo, A. and Chesnot, T. and Hallin, S. and Laval, K. and Lebeau, T. and Lemanceau, P. and Leyval, C. and Lindstr\"{o}m, K. and Pandard, P. and Romero, E. and Sarr, A. and Schloter, M. and Simonet, P. and Smalla, K. and Wilke, B.M. and Martin-Laurent, F.}, year = {2011}, pages = {454-460}, DOI = {10.1016/j.mimet.2011.01.016}, keywords = {soil DNA extraction, standardization, inter-laboratory assay, DNA fingerprint, qPCR}, journal = {Journal of microbiological methods}, doi = {10.1016/j.mimet.2011.01.016}, volume = {84}, number = {3}, issn = {0167-7012}, title = {Inter-laboratory evaluation of the ISO standard 11063 "Soil quality - Method to directly extract DNA from soil samples"}, keyword = {soil DNA extraction, standardization, inter-laboratory assay, DNA fingerprint, qPCR} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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