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Pregled bibliografske jedinice broj: 511211

LOH of PTCH1 region in BCC and ovarian carcinoma: microsatellite vs. HRM analysis


Musani, Vesna; Sabol, Maja; Car, Diana; Ozretić, Petar; Orešković, Slavko; Leović, Dinko; Levanat, Sonja
LOH of PTCH1 region in BCC and ovarian carcinoma: microsatellite vs. HRM analysis // Frontiers in bioscience (Elite ed.), E4 (2012), 3; 1049-1057 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 511211 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
LOH of PTCH1 region in BCC and ovarian carcinoma: microsatellite vs. HRM analysis

Autori
Musani, Vesna ; Sabol, Maja ; Car, Diana ; Ozretić, Petar ; Orešković, Slavko ; Leović, Dinko ; Levanat, Sonja

Izvornik
Frontiers in bioscience (Elite ed.) (1945-0494) E4 (2012), 3; 1049-1057

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
loss of heterozygosity ; high resolution melting ; PTCH1 ; basal cell carcinoma ; ovarian carcinoma

Sažetak
Loss of heterozygosity (LOH) of tumor suppressor genes is a frequent event in tumorigenesis. LOH is most often analyzed by microsatellite typing, but here we offer a fast and efficient method for simultaneous SNP genotyping and mutation scanning, which can also be used for LOH detection. High resolution melting (HRM) provides simple variant detection, and can be adopted for a wide range of applications. When a melting profile for a specific SNP is determined, the screening can be done without the need for sequencing, and only the melting profiles differing from the established melting profiles should be sequenced. LOH of PTCH1 gene is often found in a series of different tumors, for example basal cell carcinoma (BCC) and ovarian carcinoma (OC). In this study LOH was detected in 50% of BCC and 27.27% of OC, and the detection rates of microsatellite typing and HRM were comparable. Both methods depended only on the heterozygosity of the loci analyzed, but HRM offers an additional advantage of detection of all sequence variants in the gene of interest.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
098-0982464-2461 - Prijenos signala u tumorima: Hh-Gli put, interakcije i potencijalne terapije (Levanat, Sonja, MZOS ) ( POIROT)
108-1080057-0043 - Nacionalni model kliničke baze podataka u maksilofacijalnoj onkologiji (Virag, Mihajlo, MZOS ) ( POIROT)

Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Petar Ozretić (autor)

Avatar Url Vesna Musani (autor)

Avatar Url Slavko Orešković (autor)

Avatar Url Maja Sabol (autor)

Avatar Url Diana Trnski (autor)

Avatar Url Sonja Levanat (autor)

Avatar Url Dinko Leović (autor)

Citiraj ovu publikaciju

Musani, Vesna; Sabol, Maja; Car, Diana; Ozretić, Petar; Orešković, Slavko; Leović, Dinko; Levanat, Sonja
LOH of PTCH1 region in BCC and ovarian carcinoma: microsatellite vs. HRM analysis // Frontiers in bioscience (Elite ed.), E4 (2012), 3; 1049-1057 (međunarodna recenzija, članak, znanstveni)
Musani, V., Sabol, M., Car, D., Ozretić, P., Orešković, S., Leović, D. & Levanat, S. (2012) LOH of PTCH1 region in BCC and ovarian carcinoma: microsatellite vs. HRM analysis. Frontiers in bioscience (Elite ed.), E4 (3), 1049-1057.
@article{article, year = {2012}, pages = {1049-1057}, keywords = {loss of heterozygosity, high resolution melting, PTCH1, basal cell carcinoma, ovarian carcinoma}, journal = {Frontiers in bioscience (Elite ed.)}, volume = {E4}, number = {3}, issn = {1945-0494}, title = {LOH of PTCH1 region in BCC and ovarian carcinoma: microsatellite vs. HRM analysis}, keyword = {loss of heterozygosity, high resolution melting, PTCH1, basal cell carcinoma, ovarian carcinoma} }
@article{article, year = {2012}, pages = {1049-1057}, keywords = {loss of heterozygosity, high resolution melting, PTCH1, basal cell carcinoma, ovarian carcinoma}, journal = {Frontiers in bioscience (Elite ed.)}, volume = {E4}, number = {3}, issn = {1945-0494}, title = {LOH of PTCH1 region in BCC and ovarian carcinoma: microsatellite vs. HRM analysis}, keyword = {loss of heterozygosity, high resolution melting, PTCH1, basal cell carcinoma, ovarian carcinoma} }

Časopis indeksira:


  • Scopus
  • MEDLINE





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