engleski

Temporal profiling of the coding and non-coding murine cytomegalovirus transcriptomes

The global transcriptional programme of murine cytomegalovirus (MCMV) involving coding, non-coding and anti-sense transcription remains to date unknown. Here we report an oligonucleotide custom microarray platform capable of measuring both coding and non-coding transcription on a genome-wide scale. By profiling MCMV wild-type and immediate-early mutant strains in fibroblasts we find rapid activation of the transcriptome by 6.5 hours post infection with absolute dependency on ie3, but not ie1 or ie2 for genomic programming of viral gene expression. Evidence is also presented, showing for the first time, genome-wide non-coding and bi-directional transcription at late stages of MCMV infection.

transcriptome; temporal profiling; cytomegalovirus; cmv; mcmv

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