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Fourier transform infrared spectroscopic study of intact cells of the nitrogen-fixing bacterium azospirillum brasilense

The data of Fourier transform infrared (FTIR)spectroscopic measurements performed on intact cells of the soil nitrogen-fixing bacterium Azospirillum brasilense grown in a standard medium and under the conditions of an increased metal uptake are compared and discussed. The structural FTIR information obtained is considered together with atomic absorption spectrometry (AAS) data on the content of metal cations in the bacterial cells. Some methodological aspects concerning preparation of bacterial cell samples for FTIR measurements are also discussed.

Fourier transform infared spectroscopy. Azospirillum brasilense. Bacterial culture. Intact cells. Metal uptake. Atomic absorption spectrometry

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