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Pregled bibliografske jedinice broj: 509904

The effect of apigenin on cyclophosphamide and doxorubicin genotoxicity in vitro and in vivo


Bokulić, Ana; Garaj-Vrhovac, Verica; Brajša, Karmen; Đurić, Koraljka; Glojnarić, Ines; Šitum, Kristina
The effect of apigenin on cyclophosphamide and doxorubicin genotoxicity in vitro and in vivo // Journal of Environmental Science and Health A, 46 (2011), 5; 526-533 doi:10.1080/10934529.2011.551744 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 509904 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
The effect of apigenin on cyclophosphamide and doxorubicin genotoxicity in vitro and in vivo

Autori
Bokulić, Ana ; Garaj-Vrhovac, Verica ; Brajša, Karmen ; Đurić, Koraljka ; Glojnarić, Ines ; Šitum, Kristina

Izvornik
Journal of Environmental Science and Health A (1093-4529) 46 (2011), 5; 526-533

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
mutagenesis; flavonoids; apigenin micronucleus test

Sažetak
The aim of this study was to investigate the mutagenic and antigenotoxic effects of different doses of the flavonoid, apigenin, alone and in combination with the antitumor drugs, cyclophosphamide and doxorubicin, in vitro and in vivo. Using bacterial reverse mutation inhibition in vitro, with and without metabolic activation, the effect of apigenin (10 - 400 μg/plate) was studied on genotoxicity induced by cyclophosphamide (800 μg/plate) and by doxorubicin (0.2 μg/plate). Subsequent to a dose-finding study in vivo, CD1 mice were treated with either cyclophosphamide (40 mg/kg, i.p.) or doxorubicin (5 mg/kg, i.p.) with or without co-administration of apigenin (1-100 mg/kg, p.o.). Micronuclei were determined microscopically in blood smears and glutathione peroxidase (GPX), superoxide dismutase (SOD) and total antioxidative status (TAS) in whole blood, erythrocytes and plasma, respectively. Apigenin decreased doxorubicin-induced, but not cyclophosphamide-induced mutagenicity in vitro. In vivo, apigenin caused a statistically significant decrease in micronucleus frequency in response to cyclophosphamide, possibly due to active flavonoid metabolite formation or inhibition of cyclophosphamide metabolic activation. In animals treated with apigenin and doxorubicin, a significant decrease in micronucleus frequency was not observed, probably due to interindividual variability. No changes in GPX, SOD or TAS were observed in response to either cytotoxic agents or the flavonoid, possibly due to limited metabolic transformation of the drugs at the doses used. The results of the present study provide further evidence for the chemo-preventative properties of apigenin

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekti:
022-0222148-2125 - Mutageni i antimutageni u ekogenetičkim istraživanjima (Garaj-Vrhovac, Vera, MZOS ) ( POIROT)

Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb,
Fidelta d.o.o.

Citiraj ovu publikaciju

Bokulić, Ana; Garaj-Vrhovac, Verica; Brajša, Karmen; Đurić, Koraljka; Glojnarić, Ines; Šitum, Kristina
The effect of apigenin on cyclophosphamide and doxorubicin genotoxicity in vitro and in vivo // Journal of Environmental Science and Health A, 46 (2011), 5; 526-533 doi:10.1080/10934529.2011.551744 (međunarodna recenzija, članak, znanstveni)
Bokulić, A., Garaj-Vrhovac, V., Brajša, K., Đurić, K., Glojnarić, I. & Šitum, K. (2011) The effect of apigenin on cyclophosphamide and doxorubicin genotoxicity in vitro and in vivo. Journal of Environmental Science and Health A, 46 (5), 526-533 doi:10.1080/10934529.2011.551744.
@article{article, year = {2011}, pages = {526-533}, DOI = {10.1080/10934529.2011.551744}, keywords = {mutagenesis, flavonoids, apigenin micronucleus test}, journal = {Journal of Environmental Science and Health A}, doi = {10.1080/10934529.2011.551744}, volume = {46}, number = {5}, issn = {1093-4529}, title = {The effect of apigenin on cyclophosphamide and doxorubicin genotoxicity in vitro and in vivo}, keyword = {mutagenesis, flavonoids, apigenin micronucleus test} }
@article{article, year = {2011}, pages = {526-533}, DOI = {10.1080/10934529.2011.551744}, keywords = {mutagenesis, flavonoids, apigenin micronucleus test}, journal = {Journal of Environmental Science and Health A}, doi = {10.1080/10934529.2011.551744}, volume = {46}, number = {5}, issn = {1093-4529}, title = {The effect of apigenin on cyclophosphamide and doxorubicin genotoxicity in vitro and in vivo}, keyword = {mutagenesis, flavonoids, apigenin micronucleus test} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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  • Water Resources Abstracts


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