engleski

Emergence of Proteus mirabilis isolates producing TEM-52 extended-spectrum beta-lactamase in Croatia

An increased frequency of extended-spectrum β-lactamase (ESBL) positive Proteus mirabilis isolates was observed recently in Clinical Hospital Centre Split, Croatia. The aim of this study was the molecular characterization of ESBLs in P. mirabilis isolates from this hospital. Seven strains showing reduced susceptibility to ceftazidime were investigated. Antimicrobial susceptibility was determined by broth microdilution method. ESBLs were characterized by PCR and sequencing of blaESBL genes. Quinolone resistance determinants (qnr genes) were characterized by PCR. Genotyping of strains was performed by PFGE. The presence of an ESBL was confirmed in all strains by double disk synergy test. All strains were resistant to amoxycillin, piperacillin, gentamicin, ciprofloxacin, chloramphenicol, sulfamethoxazole and trimethoprim, but susceptible to ceftazidime/clavulanic acid, piperacillin/tazobactam, cefoxitin, imipenem, and meropenem PCR-sequencing using primers targeting blaESBL genes revealed TEM-52 β-lactamase. PFGE genotyping demonstrated clonal relatedness of TEM-52-producing P. mirabilis strains isolated from different clinical samples and wards within the hospital. BlaTEM-52 in three isolates was carried by a 70-kb conjugative plasmid. Our findings indicate the emergence of TEM-52 enzyme among Proteus mirabilis in Croatia.

TEM-52 beta-lactamase; Proteus mirabilis; ceftazidime

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