engleski

CAPACITY OF EXTRACTS OF CASTANEA SATIVA MILL. FOR REMOVAL OF ORGANIC, HYDROPHILIC RADICALS

The controlled production of reactive oxygen species (ROS) has an important role in various physiological processes. Unbalanced radical generation is highly correlated with many patho- physiological events such as neurodegenerative diseases, malignancy, diabetes, and atherosclerosis. ROS can react with other biomolecules, to form organic, hydrophilic radicals (e.g., ascorbil radical, semiquinone, etc.). Parts of chestnut such as: seeds, peeled chestnut, brown seed coat, leaf, catkin, spiny burs and chestnut bark have been extracted under the same conditions by 50% ethanol as an extragent and afterwards dry extracts of examined samples have been gained. The total phenolics and flavonoids content were measured using standard spectrophotometrics methods. The capacity of investigated extracts to remove radicals is evaluated by EPR method, using hydrophilic spin probe Tempone. The most efficient for removal of Tempone have shown extracts of catkin of sweet chestnut (AA = 18.1%) and brown seed coat of Grafted Italian Marrone (AA = 12.6%).

extracts; EPR; Castanea sativa Mill.; flavonoids; phenolic compounds; Tempone

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