engleski

Insulin-like growth factor 2 receptor gene Gly1619Arg polymorphism in couples with idiopathic recurrent spontaneous abortion.

Objective: The insulin-like growth factor (IGF) axis is an important regulator of fetal growth and placental development and function. Any imbalance in the IGF system leads to restriction of placental and fetal growth through restraining trophoblast invasion and decreasing placental blood flow. The insulin-like growth factor 2 receptor (IGF2R) gene is an important regulator of placental function. During pregnancy, it maintains correct levels of IGF2, whose polymorphisms were previously associated with recurrent spontaneous abortion (RSA). The aim of the study was to investigate the association between IGF2R gene Gly1619Arg G>A polymorphism and idiopathic RSA. Design and methods: A case–control study was conducted to determine the association between the IGF2R Gly1619Arg polymorphism and the risk of RSA in couples and fertile controls. The study group comprised of 149 Slovenian couples with a history of three or more consecutive spontaneous abortions of unexplained etiology before 24th week of gestation. The first control group consisted of 149 age matched unrelated women, with at least two live births, and no history of pregnancy loss while the second control group consisted of 149 age matched, unrelated, fertile men. Polymerase chain reaction and restriction fragment length polymorphism methods were performed to identify the genotypes. Results: No significant deviation from the Hardy-Weinberg equilibrium in control samples was observed. There were no statistical significant differences in genotype frequency distribution of tested genetic polymorphism: RSA women vs. female controls 3.3%/2% (AA) ; 18.8%/14.8% (AG) ; 77.9%/83.2% (GG), p=0.47 ; RSA males vs. male controls 1.3%/0.7% (AA) ; 14.1%/9.4% (AG) ; 84.6%/89.9% (GG), p=0.37. There were no statistical significant differences according to the recessive model in females with RSA compared to female healthy controls ; as well, there were no statistical significant difference in genotype frequency distribution of tested genetic polymorphism, neither according to the recessive model in males with RSA and male healthy controls ; in addition, there were no statistical significant difference in the genotype frequency distribution of tested genetic polymorphism, neither according to the recessive model or dominant model between the group of couples with RSA and control group. Conclusions: The IGF2R gene polymorphism was not associated with idiopathic RSA in Slovenian couples.

genetics; polymorphism; spontaneous abortion

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