engleski

Regio- and stereoselectivity of biotransformation of steroid hormones by recombinant human CYP enzymes

Metabolism of isomers of endogenous steroid hormones was studied in vitro using human recombinant CYP3A4 and CYP2C19 enzymes. The enzyme formats used for CYP3A4 and CYP2C19 were insect cell microsomes expressing human CYP enzymes and purified recombinant human CYP enzymes in a reconstituted system. The CYP enzyme formats incubated with steroids, testosterone, epitestosterone, androstenedione, methyltestosterone, androsterone, 5-androstane-3,17-diol, 5-androstane-3,17-diol, 5-androstane-3,17-diol, 5-dihydrotestosterone (DHT), androst-4-ene-3,17-diol and androst-4-ene-3,17-diol , produced 6-hydroxyl metabolites identified as trimethylsilyl (TMS)-ethers by a gas chromatography-mass spectrometry

steroid hormones; CYP enzymes; biotransformation; stereoselectivity; regioselectivity; in vitro

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