Isolation of Active Substances from the Seeds of the Plant Milk Thistle (Silybum marianum) and Determination of Antioxidant Activity (CROSBI ID 170099)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Gašo-Sokač, Dajana ; Kovač, Spomenka ; Bušić, Valentina
engleski
Isolation of Active Substances from the Seeds of the Plant Milk Thistle (Silybum marianum) and Determination of Antioxidant Activity
Isolation of silymarin, a mixture of well-defined flavonolignans, from the seeds of milk thistle (Silybum marianum) was achieved. Silymarin were extracted from defatted seed with acetone without heating ; the yield was 4.1 %. The antioxidant activity of silymarin was determined at ambient temperature by means of a 2, 2-diphenyl-1- picrylhydrazyl (DPPH) colorimetry with a detection scheme at 515 nm. The activity was evaluated by the decrease in absorbance as the result of a DPPH radical color change from purple to yellow. The results obtained showed that ascorbic acid was a substantially more powerful antioxidant than the silymarin, but silymarin was a significantly stronger quencher of DPPH radical than the standard silibinin.
silymarin ; silibinin ; DPPH radical scavenging ; antioxidant activity
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Podaci o izdanju
60 (9)
2011.
441-445
objavljeno
0022-9830
1334-9090
Povezanost rada
Kemija, Prehrambena tehnologija