engleski

Myogenic regulatory factors expression in rat musculus masseter after injury

Skeletal muscle is a dynamic system that responds quickly and adapts to changes in intrinsic and extrinsic stimuli. A whole repertoire of genes can be activated in response to these stimuli. The family of myogenic basic helix - loop - helix (bHLH) transcription factors (MyoD, myogenin, MRF4, Mif- 5) plays a major role in coordinating the muscle developmental program, as well as the process of adaptation. These factors contribute to the transcriptional activation of muscle genes during commitment and differentiation. The aim of this study was to determinate time course expression of MyoD and myogenin during regeneration in musculus masseter. All experiments were performed on male Sprague - Dowley rats. Histochemical, immunohistochemical and Western blot analyses were performed on masseter muscles. Regeneration process of skeletal muscles was induced by injection of bupivacain. Regeneration of masseter muscle caused a transcient upregulation of MyoD and myogenin. After reestablishment of normal muscle architecture there was no expression of MyoD and myogenin. The main conclusion of our investigation is that role of MyoD and myogenin is important in repair response of muscle fibers.

bupivacaine; masseter muscle; regulatory myogenic factors

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