engleski

A Study of Antifungal and Antiaflatoxigenic Action of Newly Synthesized Analogues of Dehydroacetic Acid

Mycelial growth and aflatoxin B1 accumulation by toxigenous mould Aspergillus flavus were inhibited by dehydroacetic acid and its newly synthesized analogues, 4-hydroxy-3-(p-toluoyl)-6-(p-tolil)-2H-pyrane-2-one (DHT) and 5-Bromo-4-hydroxy-3-(p-toluoyl)-6-(p-tolil)-2H-pyrane-2-one (BrDHT) during the growth of investigated mould on solid substrate (soy-beans). Experiments were carried out in a stationary culture at 20 oC and 30 oC, respectively, during 21 days. Mycelial growth (biomass dry weight) was determined by measuring the chitin content and concentration of aflatoxin B1 (AFB1) was quantitated by HPLC. Concentrations of DHA and DHT of 0.1 and 0.2 μg x g-1 of substrate were stimulated, but concentrations higher than 0.5 μg x g-1 of these compounds, mould growth and AFB1 accumulation partially inhibited. In experiments with 0.2 μg x g-1 of BrDHT mould growth and AFB1 accumulation were inhibited completely depending on the time and temperature of cultivation.

Toxigenous moulds; aflatoxin B1; dehydroacetic acid; chitin; HPLC

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