engleski

Phytochemical Analysis and Antimicrobial Activity of Cardaria draba L. Desv. volatiles

Two different volatile isolates from aerial parts of Cardaria draba (L.) Desv, were obtained by hydrodistillation and dichloromethane extraction and analysed by gas chromatography/gas chromatography-mass spectrometry. The main volatiles obtained by hydrodistillation originated from glucoerucin degradation, i.e. 4-(methylthio)butyl isothiocyanate (28.0%) and 5-(methylthio)pentanenitrile (13.8%). Also, volatiles without sulfur and/or nitrogen were identified, mostly hexadecanoic acid (10.8%), phytol (10.2%), dibutylphatalate (4.5%) and some other compounds in smaller percentages. Volatile isolate obtained by dichloromethane extraction after exogenous myrosinase hydrolysis contained mostly compounds originating from glucosinolate degradation. They originated from glucoraphanin (4-(methylsulfinyl)butyl isothiocyanate (69.2%) and 5-(methylsulfinyl)pentanenitrile (4.5%)) glucosinalbin (4-hydroxyphenylacetonitrile (7.2%)) and glucoerysolin (4-(methylsulfonyl)butyl isothiocyanate (5.0%)). The volatile samples were evaluated for antimicrobial activity using the disc diffusion method and determining minimum inhibitory concentrations. All volatile isolates expressed a wide range of growth inhibition activity against both Gram-positive and Gram-negative bacteria and fungi. The minimum inhibitory concentrations varied between 4 and 128 µg/ml.

Cardaria draba; Brassicaceae; glucosinolates; isothiocyanates; antimicrobial activity; GC-MS

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