engleski

Detection of heparin during F VIII isolation using improved azure A method

Cryoprecipitate with heparin was the starting material in chromatographic isolation of factor VIII (F VIII). Heparin stabilizes F VIII and in that sense enhances the recovery of F VIII during isolation. The heparin concentration during different steps of F VIII isolation procedure was determined by modified azure A method. Azure A method is a rapid and simple spectrometric method for determination of heparin following the formation of soluble complex between heparin and azure A dye. Influence of different parameters (pH, heparion concentration, stability of the dye and heparin-dye complex) on azure A method has been established. Results presented in this report show that determination of heparin is achieved by simultaneous measuring absorbance of the complex at 500 nm and azure A dye absorbance decrease at 630 nm.

F VIII; heparin; azure

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