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Pregled bibliografske jedinice broj: 499759

Co-cultivation of Legionella and amoeba Dictyostelium discoideum


Turković, Dastina; Malnar, Maja; Rebić, Danica; Trobonjača, Zlatko; Gobin, Ivana
Co-cultivation of Legionella and amoeba Dictyostelium discoideum // Liječnički vjesnik
Zagreb, Hrvatska, 2010. str. 43-43 (poster, nije recenziran, sažetak, znanstveni)


Naslov
Co-cultivation of Legionella and amoeba Dictyostelium discoideum

Autori
Turković, Dastina ; Malnar, Maja ; Rebić, Danica ; Trobonjača, Zlatko ; Gobin, Ivana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Liječnički vjesnik / - , 2010, 43-43

Skup
10th ZIMS, Zagreb International Medical Summit for students and young doctors

Mjesto i datum
Zagreb, Hrvatska, 11.-14.11.2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Legionella; amoeba; co-cultivation; Dictyostelium

Sažetak
Legionella naturally exploits numerous species of protozoa, which provide nutrients, protect the bacteria from adverse conditions and serve as a vehicle for the colonization of new habitats. D. discoideum is a unicellular, free-living amoeba that lives in soil and water and feeds on bacteria. This report describes the conditions of co-cultivation of Legionella and amoeba D. discoideum. Infection of D. discoideum with Legionella requires an incubation temperature of 25°C. Amoeba cells grown axenically were cultured in HL-5 liquid medium. Legionella were harvested after 48 h growth on BCYE plates, resuspended in water or PBS, and used to infect D. discoideum at an MOI of 10:1. Bacteria and amoeba was co-cultivated in two different media: HL5 media and assay media. The number of viable bacteria on each day was determined by measuring CFU. One day after co-cultivation significantly lower number of L. longbeachae was detected (~102) in comparisom to number of L. pneumophila (~104). The number of L. pneumophila increased during 96 hours when ~105 CFU/well was detected in bouth media. L. longbeachae was able to grew better in assay media and 96 h p.i. ~104 CFU/well was detected. In HL5 media the number of L. longbeachae increased only for 0, 5 log. Importantly, HL5 medium alone or assay media does not support L. pneumophila or L. longbeachae growth, indicating that all growth detected is a result of intracellular multiplication. In conclusion, bouth Legionella are able to infect and proliferate within D. discoideum and replication was better in assay media.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
062-0621273-1275 - Patogeneza eksperimentalne legioneloze (Miljenko Dorić, )

Ustanove
Medicinski fakultet, Rijeka