engleski

HISTOPATHOLOGICAL FINDINGS IN BROILER CHICKS TREATED WITH GIZZEROSINE

Gizzerosine is a toxic substance spontaneously produced during the heating process of fish meal. There are few studies describing the gizzard erosions (GE) induced by gizzerosine. Its pathological effects on other organs have not been described yet. In this work the pathological effects of gizzerosine on different chicken organs were investigated. The ROSS broiler chicks were divided into three groups: group A received 100% of non-medicated commercial mash for broiler chicks. In group B 50% of commercial mash was replaced with unheated fish meal (0.5 ppm gizzerosine) and in group C with heated fish meal (1.15 ppm gizzerosine) during an experimental 7-day period. Fourteen chicks from each group were sacrified every day during one week. Necropsy did not reveal notable differences between treated groups. Samples of gastrointestinal, cardiovascular and lymphoid organs, of brain and kidney were collected for histopathology analysis and, after the standard embedding and cutting, were stained with haematoxylin-eosin stain. Histopathological lesions predominantly developed in gastrointestinal organs (proventricular mucosal degeneration; edema of intestinal mucosa and lamina propria with/without multiple vacuoles; proventricular and intestinal fibroplasia of lamina propria; gizzard cuticle erosions). The most prominent were intestinal lesions; their severity increased toward the end of the trial, especially in

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