engleski

“Characteristic of glial cells of the subplate zone in diffuse and focal periventricular white matter injury”

Focal and diffuse periventricular white matter injuries (PVWMI) are the main histopathological brain lesions detected in preterm infants. Recent studies have shown that children born very preterm were more likely to develop neurological, cognitive and/or behavioral handicaps than children born preterm. Our study analysed different phenotypic expression of glial cells observed in diffuse and focal PVWMI on brain tissue (n=28) obtained from very preterm (24-30 gestational weeks - gw) and preterm (31-35 gw) infants in frontal cortex. Antibodies used: for astrocytes (Glial Fibrillary Acidic Protein- GFAP ; Monocarboxylate transporter - MCT-1 ; vimentine) ; microglia-macrophages (Ionized Calcium Binding Adapter Molecule 1 - Iba-1) ; vessels (MCT1 and CD34) and pre-oligodendrocytes (Olygodendrocyte Transcription Factor 2 - Olig2 and Myelin Basic Protein - MBP). On hematoxylin- eosin staining the different types of PVWMI were identified.The phenotypic features and morphology of glial cells in cortex (layer V/VI), superficial subplate, deep subplate and white matter below the subplate were described. Focal lesions displayed higer density of GFAP+ astrocytes in late preterms. These cells show more developed cell processes in very preterm infants vs. late preterm. Iba1 had the highest expression in the white matter, indicating activation (macrophages). Microglia was less activated in deep and superficial SP. Diffuse lesions showed actrocytes with vacuoled appearence in layers below the cortical plate in late preterms. The expression of MCT 1 in astrocytes was not different in PVWMI compared to controls, except in superficial and deep SP where it showed strong expression in vessel walls in PVWMI. This study identifies criticial differences in glial phenotypic expressions in diffuse compared to focal lesions in PVWMI. Supported by Croatian Ministry of Science grant No. 108-1081870-1876 (IK) and scholarship from the French Government (IP).

glia; subplate

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