engleski

Expression of pannexin familly of gap junction proteins in breast carcinoma

Breast cancer is the most prevailing malignancy among worldwide female population, with constantly increasing incidence and serious consequences on morbidity and mortality of affected patients. It represents genotypically and phenotypically very heterogeneous group of tumors that are diverse in their pathohistological features, expression of molecular markers, malignant/metastatic potential, clinical presentation, prognosis, and response to therapy. The purpose of this preliminary study is to determine the expression pattern of pannexin family (Panx 1, 2 and 3) of gap junction proteins in invasive ductal breast cancer in female patients. So far a total of 27 patients with invasive ductal breast cancer (diagnosed during the 2010 in Department of Pathology, University Hospital for Tumors, University Hospital “Sestre milosrdnice”, Ilica 197, Zagreb, Croatia) were included in this study. Immunohistochemical staining for pannexin 1 and 2 was performed with rabbit polyclonal anti-human pannexin-1 and pannexin-2 antibody (AbCam ab78380, AbCam ab55917) using DAB substrate and HRP reaction. Results obtained so far showed positive expression pattern for pannexin 1 and 2, both on a protein and mRNA level (data not shown). Immunohistological staining for pannexin 1 showed diffuse and granular cytoplasmatic staining with slight positivity of cell membranes in all analyzed samples while Pannexin 2 showed only diffuse cytoplasmatic staining without membrane involvement.

breast cancer ; pannexin familly ; gap junction

nije evidentirano