engleski

Detection and characterization of avian nephritis virus in ducklings

The first evidence of avian nephritis virus (ANV) in ducks is described. A diagnostic investigation was performed on the three duck farms in Croatia. Samples from ducklings dead in shell and ducklings aged 30 days were collected and prepared for molecular and histopathological examination. Intestinal and liver samples were tested by PCR for the presence of ANV, duck enteritis virus (DEV), duck hepatitis virus 1 (DHV-1) and Derzsy’s disease virus (DDV). Multiple tissues were collected for histological examination and lesions were confined to the kidney and intestine. Moderate focal interstitial and periglomerular mononuclear cell infiltrates (mostly lymphocytes and plasma cells) were detected in the kidney. Duodenum showed rather diffuse pericryptal mononuclear cell hyperplasia (lymphocytes) and fibroplasia. ANV was detected by PCR in the all intestinal samples, while no other viruses were found. Sequence comparisons of the portion of the ORF 1b encoding RNA-dependent RNA polymerase (RdRp) gene confirmed that the virus detected and sequenced from ducklings shared high nucleotide and aminoacid identities with ANV-1. Additional work is required to determine the clinicopathological significance of ANV infection in ducks.

avian nephritis virus ; astroviruses ; ducks ; sequence analysis

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