Pretražite po imenu i prezimenu autora, mentora, urednika, prevoditelja

Napredna pretraga

Pregled bibliografske jedinice broj: 489831

Genotoxicity of marine sediments in the fish hepatoma cell line PLHC-1 as assessed by the Comet assay


Šrut, Maja; Traven, Luka; Štambuk, Anamaria; Kralj, Sonja; Žaja, Roko; Mićović, Vladimir; Klobučar, Goran I.V.
Genotoxicity of marine sediments in the fish hepatoma cell line PLHC-1 as assessed by the Comet assay // Toxicology in vitro, 25 (2011), 308-314 doi:10.1016/j.tiv.2010.09.015 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 489831 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Genotoxicity of marine sediments in the fish hepatoma cell line PLHC-1 as assessed by the Comet assay

Autori
Šrut, Maja ; Traven, Luka ; Štambuk, Anamaria ; Kralj, Sonja ; Žaja, Roko ; Mićović, Vladimir ; Klobučar, Goran I.V.

Izvornik
Toxicology in vitro (0887-2333) 25 (2011); 308-314

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Comet assay; PLHC-1 cell line; marine sediments; DNA damage; extraction solvent

Sažetak
The main goal of this study was to test the usefulness of the Comet assay in the PLHC-1 hepatoma fish cell line as a tool for detecting the presence of genotoxic compounds in contaminated marine sediments. The system has been tested using both model chemicals (benzo[a]pyrene (B[a]P) and ethyl methanesulfonate (EMS)) and extracts of sediment samples obtained with solvent dichloromethane/methanol. For all of the analysed sediment extracts as well as for the model chemicals a concentration dependent genotoxic effect was observed. The sediment with the highest observed genotoxic potential was additionally extracted using various solvents in order to test which class of compounds, according to their polarity, is mostly responsible for the observed genotoxic effect. Non-polar solvents (cyclohexane and dichloromethane) yielded stronger genotoxic effect but the highest level of DNA damage was determined after exposure to sediment extract obtained with the solvent mixture dichloromethane/methanol which extracts a wide range of contaminants. Our results indicate that the PLHC-1 cell line is a suitable in vitro model in sediment genotoxicity assessment and encourage the use of fish cell lines as versatile tools in ecogenotoxicology.

Izvorni jezik
Engleski

Znanstvena područja
Biologija



POVEZANOST RADA


Projekti:
119-0982934-3110 - UČINAK ONEČIŠĆENJA NA GENETIČKU STRUKTURU ORGANIZAMA U VODENOM OKOLIŠU (Klobučar, Goran, MZOS ) ( POIROT)

Ustanove:
Prirodoslovno-matematički fakultet, Zagreb

Profili:

Avatar Url Maja Šrut (autor)

Avatar Url Luka Traven (autor)

Avatar Url Goran Klobučar (autor)

Avatar Url Roko Žaja (autor)

Avatar Url Vladimir Mićović (autor)

Avatar Url Anamaria Štambuk (autor)

Citiraj ovu publikaciju:

Šrut, Maja; Traven, Luka; Štambuk, Anamaria; Kralj, Sonja; Žaja, Roko; Mićović, Vladimir; Klobučar, Goran I.V.
Genotoxicity of marine sediments in the fish hepatoma cell line PLHC-1 as assessed by the Comet assay // Toxicology in vitro, 25 (2011), 308-314 doi:10.1016/j.tiv.2010.09.015 (međunarodna recenzija, članak, znanstveni)
Šrut, M., Traven, L., Štambuk, A., Kralj, S., Žaja, R., Mićović, V. & Klobučar, G. (2011) Genotoxicity of marine sediments in the fish hepatoma cell line PLHC-1 as assessed by the Comet assay. Toxicology in vitro, 25, 308-314 doi:10.1016/j.tiv.2010.09.015.
@article{article, author = {\v{S}rut, Maja and Traven, Luka and \v{S}tambuk, Anamaria and Kralj, Sonja and \v{Z}aja, Roko and Mi\'{c}ovi\'{c}, Vladimir and Klobu\v{c}ar, Goran I.V.}, year = {2011}, pages = {308-314}, DOI = {10.1016/j.tiv.2010.09.015}, keywords = {Comet assay, PLHC-1 cell line, marine sediments, DNA damage, extraction solvent}, journal = {Toxicology in vitro}, doi = {10.1016/j.tiv.2010.09.015}, volume = {25}, issn = {0887-2333}, title = {Genotoxicity of marine sediments in the fish hepatoma cell line PLHC-1 as assessed by the Comet assay}, keyword = {Comet assay, PLHC-1 cell line, marine sediments, DNA damage, extraction solvent} }
@article{article, author = {\v{S}rut, Maja and Traven, Luka and \v{S}tambuk, Anamaria and Kralj, Sonja and \v{Z}aja, Roko and Mi\'{c}ovi\'{c}, Vladimir and Klobu\v{c}ar, Goran I.V.}, year = {2011}, pages = {308-314}, DOI = {10.1016/j.tiv.2010.09.015}, keywords = {Comet assay, PLHC-1 cell line, marine sediments, DNA damage, extraction solvent}, journal = {Toxicology in vitro}, doi = {10.1016/j.tiv.2010.09.015}, volume = {25}, issn = {0887-2333}, title = {Genotoxicity of marine sediments in the fish hepatoma cell line PLHC-1 as assessed by the Comet assay}, keyword = {Comet assay, PLHC-1 cell line, marine sediments, DNA damage, extraction solvent} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Citati:





    Contrast
    Increase Font
    Decrease Font
    Dyslexic Font