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Pregled bibliografske jedinice broj: 489353

Stoichiometric incorporation of base substitutions at specific sites in supercoiled DNA and supercoiled recombination intermediates


Matovina, Mihaela; Seah, Nicole; Hamilton, Theron; Warren, David; Landy, Arthur
Stoichiometric incorporation of base substitutions at specific sites in supercoiled DNA and supercoiled recombination intermediates // Nucleic acids research, 38 (2010), 18; e175, 13 doi:10.1093/nar/gkq674 (međunarodna recenzija, članak, znanstveni)


CROSBI ID: 489353 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Stoichiometric incorporation of base substitutions at specific sites in supercoiled DNA and supercoiled recombination intermediates

Autori
Matovina, Mihaela ; Seah, Nicole ; Hamilton, Theron ; Warren, David ; Landy, Arthur

Izvornik
Nucleic acids research (0305-1048) 38 (2010), 18; E175, 13

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
supercoiled DNA ; base modifications

Sažetak
Supercoiled DNA is the relevant substrate for a large number of DNA transactions and has additionally been found to be a favorable form for delivering DNA and protein-DNA complexes to cells. We report here a facile method for stoichiometrically incorporating several different modifications at multiple, specific, and widely spaced sites in supercoiled DNA. The method is based upon generating an appropriately gapped circular DNA, starting from single-strand circular DNA from two phagemids with oppositely oriented origins of replication. The gapped circular DNA is annealed with labeled and unlabeled synthetic oligonucleotides to make a multiply nicked circle, which is covalently sealed and supercoiled. The method is efficient, robust and can be readily scaled up to produce large quantities of labeled supercoiled DNA for biochemical and structural studies. We have applied this method to generate dye-labeled supercoiled DNA with heteroduplex bubbles for a Förster resonance energy transfer (FRET) analysis of supercoiled Holliday junction intermediates in the λ integrative recombination reaction. We found that a higher-order structure revealed by FRET in the supercoiled Holliday junction intermediate is preserved in the linear recombination product. We suggest that in addition to studies on recombination complexes, these methods will be generally useful in other reactions and systems involving supercoiled DNA.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Biotehnologija



POVEZANOST RADA


Ustanove:
Institut "Ruđer Bošković", Zagreb

Profili:

Avatar Url Mihaela Matovina (autor)

Citiraj ovu publikaciju

Matovina, Mihaela; Seah, Nicole; Hamilton, Theron; Warren, David; Landy, Arthur
Stoichiometric incorporation of base substitutions at specific sites in supercoiled DNA and supercoiled recombination intermediates // Nucleic acids research, 38 (2010), 18; e175, 13 doi:10.1093/nar/gkq674 (međunarodna recenzija, članak, znanstveni)
Matovina, M., Seah, N., Hamilton, T., Warren, D. & Landy, A. (2010) Stoichiometric incorporation of base substitutions at specific sites in supercoiled DNA and supercoiled recombination intermediates. Nucleic acids research, 38 (18), e175, 13 doi:10.1093/nar/gkq674.
@article{article, year = {2010}, pages = {13}, DOI = {10.1093/nar/gkq674}, chapter = {e175}, keywords = {supercoiled DNA, base modifications}, journal = {Nucleic acids research}, doi = {10.1093/nar/gkq674}, volume = {38}, number = {18}, issn = {0305-1048}, title = {Stoichiometric incorporation of base substitutions at specific sites in supercoiled DNA and supercoiled recombination intermediates}, keyword = {supercoiled DNA, base modifications}, chapternumber = {e175} }
@article{article, year = {2010}, pages = {13}, DOI = {10.1093/nar/gkq674}, chapter = {e175}, keywords = {supercoiled DNA, base modifications}, journal = {Nucleic acids research}, doi = {10.1093/nar/gkq674}, volume = {38}, number = {18}, issn = {0305-1048}, title = {Stoichiometric incorporation of base substitutions at specific sites in supercoiled DNA and supercoiled recombination intermediates}, keyword = {supercoiled DNA, base modifications}, chapternumber = {e175} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


Uključenost u ostale bibliografske baze podataka:


  • BIOSIS Previews (Biological Abstracts)
  • CAB Abstracts
  • CA Search (Chemical Abstracts)
  • EMBASE (Excerpta Medica)
  • Nucleic Acids Abstracts


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