engleski

Immunohistochemical detection of p53, PCNA and Ki-67 expression in oral lichen planus in correlation with clinicopathological findings of the oral mucosa

Purpose The aim of this pilot study was to examine p53, PCNA and Ki-67 expression in oral epithelium correlated with the severity of clinicopathological alteration of oral mucosa in oral lichen planus (OLP). Method Biopsies of the oral mucosa from ten patients with clinical picture of the OLP of various clinical degrees of inflamation, hyperkeratosis and erosions were immunocytochemicaly examined for p53 and for proliferative antigens (PCNA and Ki-67) expression using the ABC method after previous pre-treatment in microwave oven. Result Marked inflammation as well as hyperkeratosis of mostly middle to strong degree was clinically observed in all patients. In cases with erosive OLP, size of erosions varied between 2 to 8 mm. The reaction of examined antigens was mosaically expressed in the epithelium; it was intranuclear and focally prominent in groups of cells. Proliferative antigen expression was also, observed in the epitithelial intracellular bridges, but not for p53. Marked cytotopographic differences were observed among examined antigens. p53 was expressed in the basal cell layer exclusively,PCNA in the basal, parabasal and spinous cell layers, and Ki-67 only in the the basal of the oral epithelium.The intensity of antigen expression varied accordingly to the degree of epithelial dysplasia. Conclusion The intensity of immunohistochemical reaction of p53, PCNA amnd Ki-67 expression was more prominent in the oral epithelium of the patients with severe clinicopathologic status of the OLP.

p53; PCNA; Ki-67; oral lichen planus

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