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An Assay in Microtitre Plates for Absolute Abundance of Chicken Interferon Alpha Transcripts (CROSBI ID 166900)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Novak Kujundžić, Renata ; Ragland, William Lauman An Assay in Microtitre Plates for Absolute Abundance of Chicken Interferon Alpha Transcripts // Acta veterinaria (Brno), 79 (2010), 3; 431-436. doi: 10.2754/avb201079030431

Podaci o odgovornosti

Novak Kujundžić, Renata ; Ragland, William Lauman

engleski

An Assay in Microtitre Plates for Absolute Abundance of Chicken Interferon Alpha Transcripts

Immunosuppression of commercial chickens is a serious animal health and economic problem in the poultry industry. The major causes of the immunosuppression are viruses that suppress transcription of interferon genes, especially interferon alpha. There is a need for monitoring of immunosuppression in commercially bred chickens. For this purpose, the absolute abundance of interferon alpha transcripts can be measured in blood of chickens by a suitable assay. Such an assay was used to estimate abundance of chicken interferon alpha in a sample of splenic cells induced with polyinosinic polycytidylic acid. The abundance measured was 29 ± 2 attomoles/µg total RNA. This assay can be performed in microtitre plates using samples collected from chickens in poultry houses.

mRNA ; absolute abundance ; poly I:C

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Podaci o izdanju

79 (3)

2010.

431-436

objavljeno

0001-7213

10.2754/avb201079030431

Povezanost rada

Biologija

Poveznice
Indeksiranost