An Assay in Microtitre Plates for Absolute Abundance of Chicken Interferon Alpha Transcripts (CROSBI ID 166900)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Novak Kujundžić, Renata ; Ragland, William Lauman
engleski
An Assay in Microtitre Plates for Absolute Abundance of Chicken Interferon Alpha Transcripts
Immunosuppression of commercial chickens is a serious animal health and economic problem in the poultry industry. The major causes of the immunosuppression are viruses that suppress transcription of interferon genes, especially interferon alpha. There is a need for monitoring of immunosuppression in commercially bred chickens. For this purpose, the absolute abundance of interferon alpha transcripts can be measured in blood of chickens by a suitable assay. Such an assay was used to estimate abundance of chicken interferon alpha in a sample of splenic cells induced with polyinosinic polycytidylic acid. The abundance measured was 29 ± 2 attomoles/µg total RNA. This assay can be performed in microtitre plates using samples collected from chickens in poultry houses.
mRNA ; absolute abundance ; poly I:C
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