engleski

Preparation of Sulfaguanidine-Imprinted Polymers for the Selective Extraction from Waters

Molecularly imprinted polymers (MIPs) are an artificially macromolecular material, which has prearrangement of structure and specific molecular recognition ability [1] because MIPs are synthesised in the presence of target molecules named templates. After removing the template, complementary binding sites are revealed, which exhibit a template-selective binding capacity. [2] In the last few years, MIPs were widely used for the selective enrichment and pretreatment of target compounds existing in complex matrix and molecular imprinting technology has been expanded to the field of environmental analysis. Sulfaguanidine belong to sulfonamide group of antibiotic which are widely used in medicine, animal husbandry and veterinary practice. Because of sulfaguanidine physicochemical properties and great production and consumption in Croatia, especially in veterinary practice, preparation of molecularly imprinted polymers with sulfaguanidine as a template molecule is a big challenge. In this study the preparation of molecularly imprinted (MIP) and non-imprinted polymers (NIP) for solid-phase extraction (SPE) of sulfaguanidine from water samples (pH value 4.00) was presented. For that purpose we used different functional monomers (methacrylic acid, 2-hydroxyethyl methacrylate and 4-vinyl-pyridine), cross-linker (ethylene-glycol-dimethacrylate) and initiator (2, 2’- azobis isobutyronitrile). Retained sulfaguanidine was eluted from the sorbent with adequate volumes of MeOH:HAc (9:1) and later with pure methanol. Efficiency of sulfaguanidine elution was checked by 3-aminophenol reagent. The solid-phase extraction efficiency and efficiency of prepared MIP sorbents were determined by thin-layer chromatography. It has been determined that the highest extraction efficiency of sulfaguanidine was realised with molecularly imprinted polymer based on methacrylic acid. References [1] X. Jiang, W. Tian, C. Zhao, H. Zhang, M. Liu, Talanta 2007, 72, 119–125. [2] N. Zheng, Q. Fu, Y.-Z Li, W-B Chang, Z.-M. Wang, T.-J. Li, Microchemical Journal 2001, 69, 153-158.

molecularly imprinted polymers; sulfaguanidine; thin-layer chromatography

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