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Advances in methods for the determination of biologically relevant lipid peroxidation products (CROSBI ID 166764)

Prilog u časopisu | pregledni rad (znanstveni) | međunarodna recenzija

Spickett, Corinne M ; Wiswedel, Ingrid ; Siems, Werner ; Žarković, Kamelija ; Žarković, Neven Advances in methods for the determination of biologically relevant lipid peroxidation products // Free radical research, 44 (2010), 10; 1172-1202. doi: 10.3109/10715762.2010.498476

Podaci o odgovornosti

Spickett, Corinne M ; Wiswedel, Ingrid ; Siems, Werner ; Žarković, Kamelija ; Žarković, Neven

engleski

Advances in methods for the determination of biologically relevant lipid peroxidation products

Lipid peroxidation is recognized to be an important contributor to many chronic diseases, especially those of an infl ammatory pathology. In addition to their value as markers of oxidative damage, lipid peroxidation products have also been shown to have a wide variety of biological and cell signalling effects. In view of this, accurate and sensitive methods for the measurement of lipid peroxidation products are essential. Although some assays have been described for many years, improvements in protocols are continually being reported and, with recent advances in instrumentation and technology, highly specialized and informative techniques are increasingly used. This article gives an overview of the most currently used methods and then addresses the recent advances in some specifi c approaches. The focus is on analysis of oxysterols, F 2 -isoprostanes and oxidized phospholipids by gas chromatography or liquid chromatography mass spectrometry techniques and immunoassays for the detection of 4-hydroxynonenal

oxidative stress; oxysterols; F2 isoprostanes; 4-hydroxynonenal; aldehydes; mass spectroscopy; ELISA; immunohystochemistry

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Podaci o izdanju

44 (10)

2010.

1172-1202

objavljeno

1071-5762

10.3109/10715762.2010.498476

Povezanost rada

Kemija, Temeljne medicinske znanosti, Biologija

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