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Pregled bibliografske jedinice broj: 488010

An inter-laboratory validation of methods of lipid peroxidation measurement in UVA-treated human plasma samples


Breusing, Nicolle; Grune, Tilman; Andrišić, Luka; Atalay, Mustafa; Bartosz, Grzegorz; Biasi, Fiorella; Borović, Suzana; Bravo, Laura; Casals, Isidre; Casillas, Rosario et al.
An inter-laboratory validation of methods of lipid peroxidation measurement in UVA-treated human plasma samples // Free radical research, 44 (2010), 10; 1203-1215 doi:10.3109/10715762.2010.499907 (međunarodna recenzija, pregledni rad, znanstveni)


Naslov
An inter-laboratory validation of methods of lipid peroxidation measurement in UVA-treated human plasma samples

Autori
Breusing, Nicolle ; Grune, Tilman ; Andrišić, Luka ; Atalay, Mustafa ; Bartosz, Grzegorz ; Biasi, Fiorella ; Borović, Suzana ; Bravo, Laura ; Casals, Isidre ; Casillas, Rosario ; Dinischiotu, Anca ; Drzewinska, Joanna ; Faber, Heidemarie ; Mohd Fauzi, Norsyahida ; Gajewska, Agnieszka ; Gambini, Juan ; Gradinaru, Daniela ; Kokkola, Tarja ; Lojek, Antonin ; Luczaj, Wojciech ; Margina, Denisa ; Mascia, Cinzia ; Mateos, Raquel ; Meinitzer, Andreas ; Mitjavila, María Teresa ; Mrakovčić, Lidija ; Munteanu, Maria Cristina ; Podborska, Martina ; Poli, Giuseppe ; Sicinska, Paulina ; Skrzydlewska, Elzbieta ; Vina, Jose ; Wiswedel, Ingrid ; Žarković, Neven ; Zelzer, Sieglinde ; Spickett, Corinne M.

Izvornik
Free radical research (1071-5762) 44 (2010), 10; 1203-1215

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, pregledni rad, znanstveni

Ključne riječi
Oxidative stress; F2-Isoprostanes; 4.-hydroxynonenal; malondialdehyde

Sažetak
Lipid peroxidation products like malondialdehyde, 4-hydroxynonenal and F 2 -isoprostanes are widely used as markers of oxidative stress in vitro and in vivo . This study reports the results of a multi-laboratory validation study by COST Action B35 to assess inter-laboratory and intra-laboratory variation in the measurement of lipid peroxidation. Human plasma samples were exposed to UVA irradiation at different doses (0, 15 J, 20 J), encoded and shipped to 15 laboratories, where analyses of malondialdehyde, 4-hydroxynonenal and isoprostanes were conducted. The results demonstrate a low within-day-variation and a good correlation of results observed on two different days. However, high coeffi cients of variation were observed between the laboratories. Malondialdehyde determined by HPLC was found to be the most sensitive and reproducible lipid peroxidation product in plasma upon UVA treatment. It is concluded that measurement of malondialdehyde by HPLC has good analytical validity for inter-laboratory studies on lipid peroxidation in human EDTA-plasma samples, although it is acknowledged that this may not translate to biological validity.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biologija, Temeljne medicinske znanosti



POVEZANOST RADA


Projekt / tema
098-0982464-2519 - Lipidi, slobodni radikali i njihovi glasnici u integrativnoj onkologiji (Neven Žarković, )

Ustanove
Institut "Ruđer Bošković", Zagreb

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE


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