Modulation of ROS production in human leukocytes by ganglioside micelles (CROSBI ID 166639)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Gavella, Mirjana ; Kveder, Marina ; Lipovac, Vaskresenija
engleski
Modulation of ROS production in human leukocytes by ganglioside micelles
Recent studies have reported that exogenous gangliosides, the sialic acid containing glycosphingolipids, are able to modulate many cellular functions. We examined the effect of micelles of mono- and trisialoganglioside GM1 and GT1b on the production of reactive oxygen species by stimulated human polymorphonuclear neutrophils using different spectroscopic methods. The results indicated that exogenous gangliosides did not influence extracellular superoxide anion (O2.-) generation by polymorphonuclear neutrophils activated by receptor-dependent formyl-methionyl-leucyl-phenylalanine. However, when neutrophils were stimulated by receptor-bypassing phorbol 12-myristate 13-acetate (PMA), gangliosides above their critical micellar concentrations prolonged the lag time preceding the production in a concentration-dependent way, without affecting total extracellular O2.- generation detected by superoxide dismutase-inhibitable cytochrome c reduction. The effect of ganglioside GT1b (100 µM) on increase in lag time was found to be significant by means of both superoxide dismutase-inhibitable cytochrome c reduction assay and EPR spectroscopy (P<0.0001 and P<0.005, respectively). The observed phenomena can be attributed to the ability of ganglioside micelles, attached to the cell surface, to slow down PMA uptake, thus increasing the diffusion barrier and consequently delaying membrane events responsible for PMA-stimulated O2.- production.
ganglioside micelles; reactive oxygen species; human polymorphonuclear neutrophils
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
nije evidentirano
Podaci o izdanju
43 (10)
2010.
942-949
objavljeno
0100-879X
10.1590/S0100-879X2010007500092
Povezanost rada
Fizika, Temeljne medicinske znanosti, Biologija