Intraspecies variability in Vipera ammodytes ammodytes venom related to its toxicity and immunogenic potential (CROSBI ID 166513)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Halassy, Beata ; Brgles, Marija ; Habjanec, Lidija ; Lang Balija, Maja ; Kurtović, Tihana ; Marchetti-Deschmann, Martina ; Križaj, Igor ; Allmaier, Guenter
engleski
Intraspecies variability in Vipera ammodytes ammodytes venom related to its toxicity and immunogenic potential
Vipera ammodytes is the most venomous European snake, whose venom has been used as antigen for immunization of antivenom-producing animals. Same as venom of any other snake, it is a complex mixture of proteins, peptides and other compounds which biochemical and pharmacological variability has been demonstrated at inter- and intraspecies level. In this work we demonstrated intraspecific variability between 8 venom production batches using both the conventional and the new methodology. Moreover, in contrast to literature on different venoms’ variability, for the first time we were able to select those biochemical differences that are related to and give information on the venom’s toxicity and immunogenicity. We have shown that methods quantifying ammodytoxin (the most toxic compound identified so far in the Vipera ammodytes ammodytes venom) content of the venom clearly distinguish between high and low immunogenic venoms.
Vipera ammodytes ammodytes venom; Ammodytoxin; Monolithic columns; Capillary electrophoresis-on-a-chip; ELISA; Lethal toxicity
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Podaci o izdanju
153 (2)
2011.
223-230
objavljeno
1532-0456
10.1016/j.cbpc.2010.10.007
Povezanost rada
Kemija, Temeljne medicinske znanosti, Biotehnologija