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Partial characterization of the molecular nature of collagen-linked fluorescence: role of diabetes and end-stage renal disease (CROSBI ID 567146)

Prilog sa skupa u zborniku | sažetak izlaganja sa skupa

Sell, David R. ; Nemet, Ina ; Monnier, Vincent M. Partial characterization of the molecular nature of collagen-linked fluorescence: role of diabetes and end-stage renal disease // Abstracts of 10th International Symposium on the Maillard Reaction / - (ur.). Palm Cove: IMARS, 2009. str. 113-x

Podaci o odgovornosti

Sell, David R. ; Nemet, Ina ; Monnier, Vincent M.

engleski

Partial characterization of the molecular nature of collagen-linked fluorescence: role of diabetes and end-stage renal disease

Protein-linked long-wavelength fluorescence at excitation/emission (ex/em) 370/440 nm (LW) has commonly been used as a measure of the Maillard reaction in vivo, but can form nonspecifically from other reactions as well as other than sugars. Recently, a pragmatic interest has been developed into elucidating the chemical nature of LW due to the introduction onto the commercial market of diagnostic devices capable of noninvasively measure LW in human forearm skin as a predictor for diabetes and its complications. Here, we initiated studies into the structure of a fluorophore (LW1) identified as a single major fluorescent peak in HPLC chromatograms from enzymatic digests of 75 g human skin obtained at autopsy from patients with diabetes and ESRD. Partial characterization of LW1 showed a UV maximum at 348 nm, fluorescence maxima at ex/em 348/465 nm, an accurate exact mass of 623.2746 ± 0.0017 Da, and daughter ions at m/z 447 > 318 > 402 > 494 by ESI-LC/MS. NMR experiments including 1H-NMR, TOCSY, and HETCOR suggest the presence of the epsilon protons of the lysine together with sugar molecules in a hetero-aromatic ring-type structure. As assayed by LC/MS-SRM ; i.e., selective reaction monitoring for parent to daughter ion at m/z 623 to 447, LW1 significantly increased with age (P<0.0001) and was strongly catalyzed by diabetes (P<0.0001) and end-stage renal disease (ESRD, P=0.021). LW1 was also detected in human serum protein samples from patients with diabetes and ESRD as monitored by either LC/fluorescence or LC/MS. Because LW can be measured noninvasively in skin as well as in sera, this research may have broad implications for the development of novel methodologies for the assessment of risk for macrovascular disease progression in diabetes.

Fluorescence; Maillard reaction; Diabetes; NMR; LC/MS-SRM

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Podaci o prilogu

113-x.

2009.

objavljeno

Podaci o matičnoj publikaciji

Abstracts of 10th International Symposium on the Maillard Reaction

-

Palm Cove: IMARS

Podaci o skupu

10th International Symposium on the Maillard Reaction

poster

29.08.2009-01.09.2009

Cairns, Australija

Povezanost rada

Kemija