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Bioethanol production on sugar beet pulp

The bioethanol production on sugar beet pulp (SBP) can be divided into three main steps: SBP pretreatment, fermentation and ethanol recovery. In this research, different pretreatment methods were used to hydrolyze celluloses and pectin fractions of dried sugar beet pulp into fermentable sugars. Comparison between enzymes and acid pretreatment methods pointed out that higher sugars production efficiency was achieved by enzymes pretreatment method (pH 3.5-5 and temperature 30-50oC). Different combinations (ratio and concentration) of proteases, cellulases and pectinases were examined in order to define the most suitable enzymes combination for hydrolysis of SPB. In this research, for fermentation of sugar beet pulp Saccharomyces cerevisiae was used. During fermentation of untreated SBP ethanol yield was only 0.3 g/L. In fermentation process of enzymes pretreated SBP at 40oC and pH 4.8 with the most suitable enzymes combination considerable increase of ethanol yield (8 g/L) was observed. At the same time, the conversion rate of fermentable sugars into ethanol was 0.13 g ethanol/g dry weight SBP.

Bioethanol; Saccharomyces cerevisiae; enzymes and acid pretreatment; sugar beet pulp

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