engleski

Molecular genetics of myocardial infarction

Coronary heart disease (CHD), and consequently myocardial infarction (MI), is a multifactorial disease, influenced by biochemical and genetic factors. Lipoprotein molecular markers, Lp(a) and apo E, together with new candidates associated with CHD by unknown mechanisms such as the angiotensin converting enzyme (ACE) polymorphism, have been ivnestigated to understand the factors underlying the genetic basis of this disorder. In this study, 60 patients with MI and controls were studied for apo E genotype, ACE gene typing and Lp(a) levels. Blood was obtained one to three months after myocardial infarction event. Apo E gene type was determined using restriction endonuclease HhaI isotyping. ACE insertion/deletion polymorphism was analyzed also by PCR with oligonucleotide flanking the polymorphic region of intron 16 of the ACE gene. Results revealed 20% of the patients to be E4/4 homozygotes indicating an increased prevalence of E4/4 homozygosity as compared to control group (2%), and suggesting its probable atherogenic role in IM. Results of ACE genotypes showed 44% of the study patients to be homozygous for DD allele, 24% for II and 32% for ID allele. In control subjects, the following genotypes were observed (%): II=24, ID=52, DD=24. The study also demonstrated a positive correlation between apoE 4/4 and the level of plasma Lp(a), cholesterol, LDL cholesterol and DD allele. This finding might prove useful in the detection of patients most probably susceptible to the development of coronary heart disease.

myocardial infarction; coronary heart disease

nije evidentirano