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Influence of repeated inhalation anaesthasia on the number of blood cells and biochemical parameters of mice

The toxicity of inhalation anaesthetics is related to the metabolite rate and highly reactive metabolit products. Study compared the influence of repeated sevoflurane, halothane and isoflurane anaesthesia on the number of peripheral blood cells and biochemical parameters of Swiss albino mice. Study was performed in accordance with the Guidelines for the Care and Use of Laboratory Animals, approved by the Ethical Committee of Medical School. Control, sevoflurane, halothane and isoflurane groups of mice were exposed to repeated anaesthesia two hours daily for three consecutive days. Anaesthesia was maintained with sevoflurane (2, 4 vol%), halothane (1, 5 vol%) or isoflurane (1, 7 vol%) in oxygen/air mixture 50:50. The samples of blood from axillary vein of mice were analysed with standard laboratory methods. Data (mean±SD) are shown in the Table 1.Repeated anaesthesia with halothane induced significant increase (p<0, 05) of the rate of mononuclear cells compared with sevoflurane, and serum bilirubin (p<0.005) compared with control group of mice. Activity of liver enzymes were changed, halothane increases AST, ALT and LD levels. Isoflurane increased urea levels after repeated exposure (p<0.05 compared with all groups) but creatinine levels were significantly reduced. Repeated inhalation anaesthesia induced changes in laboratory findings of mice.

sevoflurane; halothane and isoflurane; mice

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