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Pregled bibliografske jedinice broj: 483909

HPLC-DAD-MS-MS Monitoring of Veterinary Pharmaceuticals in Water after Degradation by Fenton Process and Toxicological Evaluation


Ašperger Danijela; Djurić Ivana; Vujević Dinko; Pelko Sanja; Periša Martina; Babić Sandra; Horvat Alka J.M.; Koprivanac Natalija; Kaštelan- Macan Marija
HPLC-DAD-MS-MS Monitoring of Veterinary Pharmaceuticals in Water after Degradation by Fenton Process and Toxicological Evaluation // ISC 2010 - 28TH INTERNATIONAL SYMPOSIUM ON CHROMATOGRAPHY / Yolanda Picó | U. Valencia (ur.).
Valencia, 2010. str. 635-635 (poster, međunarodna recenzija, sažetak, znanstveni)


Naslov
HPLC-DAD-MS-MS Monitoring of Veterinary Pharmaceuticals in Water after Degradation by Fenton Process and Toxicological Evaluation
(HPLC-DAD-MS-MS MONITORING OF VETERINARY PHARMACEUTICALS IN WATER AFTER DEGRADATION BY FENTON PROCESS AND TOXICOLOGICAL EVALUATION)

Autori
Ašperger Danijela ; Djurić Ivana ; Vujević Dinko ; Pelko Sanja ; Periša Martina ; Babić Sandra ; Horvat Alka J.M. ; Koprivanac Natalija ; Kaštelan- Macan Marija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
ISC 2010 - 28TH INTERNATIONAL SYMPOSIUM ON CHROMATOGRAPHY / Yolanda Picó | U. Valencia - Valencia, 2010, 635-635

Skup
ISC 2010 - 28TH INTERNATIONAL SYMPOSIUM ON CHROMATOGRAPHY

Mjesto i datum
Valencia, Španjolska, September, 12-16, 2010

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Pharmaceuticals; fenton process; toxicity; HPLC-DAD-MS-MS

Sažetak
Pharmaceuticals, both in their parent form and as metabolites, are commonly released into the environment at trace levels through conventional sewage treatment plants. Another source of entry of pharmaceuticals in the aquatic environment is a result of its veterinary use, mainly in aquaculture practices. Pharmaceuticals have been detected in ground, surface, drinking, tap and ocean water. Pharmaceuticals released in the environment may impose toxicity on any level of the biological hierarchy. In addition to toxic effects, certain classes of pharmaceuticals may cause long-term and irreversible change to the micro-organisms genome, making them resistant in their presence, even at low concentrations. It is therefore important to develop efficient treatment methodologies for limiting the presence of pharmaceuticals contaminants in aquatic environments. Presence of residual pharmaceuticals in the environment and especially in aquatic systems in particular represents a serious environmental problem as these compounds could be resistant to biological degradation processes and usually escape intact from conventional treatment plants, may impose serious toxic and other effects to humans and other living organisms. Since such compounds have been found to be present at minute concentrations, more sophisticated and laborious analytical tools for their accurate determination are required. Therefore, it is not surprising that research has recently been directed towards the application of non-biological processes for the destruction of pharmaceuticals in water systems with the emphasis on advanced oxidation processes (AOPs). [1, 2] This work has tackled degradation of fluoroquinolone antibiotic ciprofloxacin, glucocorticosteroid dexamethasone, anthelmintic febantel, sulfonamide antibiotic sulfamethoxazole and their synergist trimethoprim in water by Fenton process at lab scale. Aqueous solutions of pharmaceuticals were prepared by dissolving of appropriate amount of investigated compounds in double deionised water at an initial concentration of 1 and 10 mg/L. After 30 minutes of Fenton process samples of reaction mixture were subjected to TOC and HPLC-DAD analysis in order to determine mineralization i.e. removal extent of particular pharmaceutical. Furthermore, identification of the intermediate products generated during the process were performed using the liquid chromatography coupled to mass spectrometry (HPLC-ESI(+)-MS-MS). Chromatographic conditions include gradient separation with 0.01% formic acid in water and 0.01% formic acid in acetonitrile at 30 oC on Synergi 4 μ Fusion-RP 80 Å column (150x2.0 mm for HPLC-MS-MS and 150x4.6 mm for HPLC-DAD). The evaluation of toxicity by measuring the bioluminescence inhibition of Vibrio fischeri bioassays during the Fenton process was also performed.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biotehnologija



POVEZANOST RADA


Projekt / tema
125-1253008-1350 - Razvoj naprednih analitičkih metoda za određivanje farmaceutika u okolišu (Sandra Babić, )
125-2120898-3148 - Hrvatsko nazivlje u analitičkoj kemiji (Marija Kaštelan-Macan, )
UKF 5/207

Ustanove
Fakultet kemijskog inženjerstva i tehnologije, Zagreb